Literature summary for 1.4.3.19 extracted from

Weiss, M.S.; Pavlidis, I.V.; Vickers, C.; Hoehne, M.; Bornscheuer, U.T.
Glycine oxidase based high-throughput solid-phase assay for substrate profiling and directed evolution of (R)- and (S)-selective amine transaminases (2014), Anal. Chem., 86, 11847-11853 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Geobacillus kaustophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-iminoacetate + H2O	Geobacillus kaustophilus	-	glyoxylate + NH3	-	?
glycine + H2O + O2	Geobacillus kaustophilus	-	glyoxylate + NH3 + H2O2	-	?
glycine + O2	Geobacillus kaustophilus	-	2-iminoacetate + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Geobacillus kaustophilus	Q5L2C2	-	-

Purification (Commentary)

Purification (Comment)	Organism
His-Trap column chromatography, and Superdex 200 gel filtration	Geobacillus kaustophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-iminoacetate + H2O	-	Geobacillus kaustophilus	glyoxylate + NH3	-	?
glycine + H2O + O2	-	Geobacillus kaustophilus	glyoxylate + NH3 + H2O2	-	?
glycine + O2	-	Geobacillus kaustophilus	2-iminoacetate + H2O2	-	?

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Release 2023.1 (January 2023)