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Literature summary for 1.4.3.19 extracted from

  • Sehanobish, E.; Williamson, H.R.; Davidson, V.L.
    Roles of conserved residues of the glycine oxidase GoxA in controlling activity, cooperativity, subunit composition, and cysteine tryptophylquinone biosynthesis (2016), J. Biol. Chem., 291, 23199-23207 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.3.19

Protein Variants

Protein Variants Comment Organism
F237A the mutant shows stronlgy reduced catalytic efficiency compared to the wild type enzyme Marinomonas mediterranea
F237Y the mutant shows reduced catalytic efficiency compared to the wild type enzyme Marinomonas mediterranea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.416
-
 O2 mutant enzyme F237A, at 30°C, pH not specified in the publication Marinomonas mediterranea
0.826
-
 glycine mutant enzyme F237A, at 30°C, pH not specified in the publication Marinomonas mediterranea
2.27
-
 O2 mutant enzyme F237Y, at 30°C, pH not specified in the publication Marinomonas mediterranea
2.6
-
 O2 wild type enzyme, at 30°C, pH not specified in the publication Marinomonas mediterranea
3.03
-
 glycine mutant enzyme F237Y, at 30°C, pH not specified in the publication Marinomonas mediterranea
39
-
 glycine wild type enzyme, at 30°C, pH not specified in the publication Marinomonas mediterranea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-iminoacetate + H2O Marinomonas mediterranea
-
 glyoxylate + NH3
-
 ?
glycine + H2O + O2 Marinomonas mediterranea
-
 glyoxylate + NH3 + H2O2
-
 ?
glycine + O2 Marinomonas mediterranea
-
 2-iminoacetate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Marinomonas mediterranea
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-iminoacetate + H2O
-
 Marinomonas mediterranea glyoxylate + NH3
-
 ?
glycine + H2O + O2
-
 Marinomonas mediterranea glyoxylate + NH3 + H2O2
-
 ?
glycine + O2
-
 Marinomonas mediterranea 2-iminoacetate + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
GoxA
-
 Marinomonas mediterranea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
 O2 mutant enzyme F237A, at 30°C, pH not specified in the publication Marinomonas mediterranea
15
-
 O2 mutant enzyme F237Y, at 30°C, pH not specified in the publication Marinomonas mediterranea
39
-
 glycine wild type enzyme, at 30°C, pH not specified in the publication Marinomonas mediterranea
93
-
 O2 wild type enzyme, at 30°C, pH not specified in the publication Marinomonas mediterranea

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
 O2 mutant enzyme F237A, at 30°C, pH not specified in the publication Marinomonas mediterranea
6.6
-
 O2 mutant enzyme F237Y, at 30°C, pH not specified in the publication Marinomonas mediterranea
36
-
 O2 wild type enzyme, at 30°C, pH not specified in the publication Marinomonas mediterranea