Literature summary for 1.4.3.19 extracted from

Qin, Y.; Wu, G.; Guo, Y.; Ke, D.; Yin, J.; Wang, D.; Fan, X.; Liu, Z.; Ruan, L.; Hu, Y.
Engineered glyphosate oxidase coupled to spore-based chemiluminescence system for glyphosate detection (2020), Anal. Chim. Acta, 1133, 39-47 .

Search for more literature for 1.4.3.19

Application

Application	Comment	Organism
analysis	development of a chemiluminescence biosensor for glyphoste detection by coupling the engineered enzyme to a spore-based system for H2O2 detection. The chemiluminescence intensity is linearly proportional to the glyphosate concentration within 0.09-75.00 mg/l	Bacillus cereus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Bacillus cereus

Protein Variants

Protein Variants	Comment	Organism
D103Y/D121G/S122P/R238K/K367R/T368R	mutant displays increased activity towards glyphosate with dramatic loss of activity with glycine	Bacillus cereus
S122P/H164R/Q346R/E357G/K367R/T368R	mutant displays increased activity towards glyphosate with dramatic loss of activity with glycine	Bacillus cereus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.051	-	glyphosate	mutant D103Y/D121G/S122P/R238K/K367R/T368R, pH 7, 25°C	Bacillus cereus
0.061	-	glyphosate	mutant S122P/H164R/Q346R/E357G/K367R/T368R, pH 7, 25°C	Bacillus cereus
1.04	-	glycine	wild-type, pH 7, 25°C	Bacillus cereus
33.47	-	glycine	mutant S122P/H164R/Q346R/E357G/K367R/T368R, pH 7, 25°C	Bacillus cereus
59.94	-	glycine	mutant D103Y/D121G/S122P/R238K/K367R/T368R, pH 7, 25°C	Bacillus cereus
84.79	-	glyphosate	wild-type, pH 7, 25°C	Bacillus cereus

Organism

Organism	UniProt	Comment	Textmining
Bacillus cereus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glycine + H2O + O2	-	Bacillus cereus	glyoxylate + NH3 + H2O2	-	?
glyphosate + H2O + O2	-	Bacillus cereus	? + H2O2	-	?

Subunits

Subunits	Comment	Organism
?	x * 42000, SDS-PAGE	Bacillus cereus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.095	-	glyphosate	wild-type, pH 7, 25°C	Bacillus cereus
0.103	-	glycine	mutant D103Y/D121G/S122P/R238K/K367R/T368R, pH 7, 25°C	Bacillus cereus
0.136	-	glycine	wild-type, pH 7, 25°C	Bacillus cereus
0.158	-	glycine	mutant S122P/H164R/Q346R/E357G/K367R/T368R, pH 7, 25°C	Bacillus cereus
0.162	-	glyphosate	mutant D103Y/D121G/S122P/R238K/K367R/T368R, pH 7, 25°C	Bacillus cereus
0.19	-	glyphosate	mutant S122P/H164R/Q346R/E357G/K367R/T368R, pH 7, 25°C	Bacillus cereus

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Release 2023.1 (January 2023)