Any feedback?
Literature summary for 1.4.3.2 extracted from
- Isolation and characterization of an unusual form of L-amino acid oxidase from King cobra (Ophiophagus hannah) venom (1989), Biochem. Int., 19, 937-944.
General Stability
| General Stability | Organism |
|---|---|
| freezing, quick freezing with dry ice/acetone mixture and storage at -15°C for 60 h, enzyme is stable | Ophiophagus hannah |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | substrate inhibition | Ophiophagus hannah |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
L-leucine | - |
Ophiophagus hannah |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
2 * 68000, SDS-PAGE | Ophiophagus hannah |
| 140000 | - |
gel filtration | Ophiophagus hannah |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ophiophagus hannah | - |
king cobra | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 3.8% carbohydrate | Ophiophagus hannah |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ophiophagus hannah |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Ophiophagus hannah | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 7.4, 20 mM Tris-HCl, 3 months | Ophiophagus hannah |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-amino acid + H2O + O2 | - |
Ophiophagus hannah | 2-oxo acid + NH3 + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 68000, SDS-PAGE | Ophiophagus hannah |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
3 months, no loss of activity | Ophiophagus hannah |
| 25 | - |
1 month, no loss of activity | Ophiophagus hannah |
| 37 | - |
5 days, no loss of activity, 14 days, 20% loss of activity | Ophiophagus hannah |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 86.6 | - |
L-leucine | - |
Ophiophagus hannah | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Ophiophagus hannah |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
25°C, 1 h stable | Ophiophagus hannah |
| 9 | - |
25°C, 5 min, 47% loss of activity, complete loss of activity after 30 min | Ophiophagus hannah |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 2 mol of FAD per mol of enzyme | Ophiophagus hannah | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
