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Literature summary for 1.4.3.2 extracted from

  • Geueke, B.; Hummel, W.
    Heterologous expression of Rhodococcus opacus L-Amino acid oxidase of Streptomyces lividans (2003), Protein Expr. Purif., 28, 303-309.
    View publication on PubMed
Search for more literature for 1.4.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Streptomyces lividans Rhodococcus opacus
expression into different Escherchia coli and Streptomyces lividans vectors. Expression in Escherichia coli results in an insoluble protein whereas Steptomyces lividans is a suitable host for the heterologous production of L-AAO Rhodococcus opacus

Inhibitors

Inhibitors Comment Organism Structure
L-alanine
-
 Rhodococcus opacus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
 L-alanine wild-type enzyme, 30°C, pH 7.6 Rhodococcus opacus
0.45
-
 L-alanine recombinant enzyme, expressed in Streptomyces lividans, 30°C, pH 7.6 Rhodococcus opacus

Organism

Organism UniProt Comment Textmining
Rhodococcus opacus
-
-
-
Rhodococcus opacus Q8VPD4 lao-gene; DSM 43250 strain
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and recombinant enzyme Rhodococcus opacus
wild-type, 76fold, recombinant L-AAO expressed in and Streptomyces lividans, 186fold Rhodococcus opacus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4
-
 wild-type enzyme: 76fold purification, recombinant enzyme, expressed in and Streptomyces lividans: 186fold purification, 30°C, pH 7.6 Rhodococcus opacus
5.4
-
 wild-type and recombinant enzyme Rhodococcus opacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + H2O + O2
-
 Rhodococcus opacus 2-oxopropanoic acid + NH3 + H2O2
-
 ?
L-alanine + H2O + O2 highly enantioselective enzyme with a broad substrate specificity Rhodococcus opacus 2-oxopropanoic acid + NH3 + H2O2
-
 ?
L-alanine + H2O + O2
-
 Rhodococcus opacus pyruvate + NH3 + H2O2
-
 r

Synonyms

Synonyms Comment Organism
L-AAO
-
 Rhodococcus opacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rhodococcus opacus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.052 2.1 L-alanine wild-type enzyme Rhodococcus opacus
0.057 0.65 L-alanine recombinant enzyme, expressed in and Streptomyces lividans, 30°C, pH 7.6 Rhodococcus opacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Rhodococcus opacus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information wild-type L-AAO, no substrate inhibition up to 0.5 M L-alanine Rhodococcus opacus
221
-
 L-alanine recombinant L-AAO, expressed in and Streptomyces lividans, 30°C, pH 7.6 Rhodococcus opacus