Literature summary for 1.4.3.2 extracted from

Georgieva, D.; Kardas, A.; Buck, F.; Perbandt, M.; Betzel, C.
Isolation, crystallization and preliminary X-ray diffraction analysis of L-amino-acid oxidase from Vipera ammodytes ammodytes venom (2008), Acta Crystallogr. Sect. F, 64, 918-921.

Search for more literature for 1.4.3.2

Crystallization (Commentary)

Crystallization (Comment)	Organism
native isozyme LAAO I from venom, sitting drop vapour-diffusion method, mixing of 0.001 ml of protein solution with 10 mg/ml protein with 0.001 ml of a precipitant solution consisting of 16% w/v PEG 3350 and 0.04 M zinc acetate against 1 ml of reservoir solution at 20°C for 2 days, X-ray diffraction structure determination and analysis at 2.6 A resolution	Vipera ammodytes ammodytes

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	venom	Vipera ammodytes ammodytes	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	x * 55000, LAAO I, SDS-PAGE	Vipera ammodytes ammodytes

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
an L-amino acid + H2O + O2	Vipera ammodytes ammodytes	L-amino-acid oxidase catalyzes the oxidative deamination of a number of L-amino acids to their respective imino acids with concomitant hydrolysis to alpha-keto acids, together with the production of ammonia and hydrogen peroxide	a 2-oxo-acid + NH3 + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Vipera ammodytes ammodytes	-	captured in northwest Bulgaria, five LAAO isozymes	-

Purification (Commentary)

Purification (Comment)	Organism
native isozymes from venom by 2times anion exchange chromatography and dialysis to homogeneity	Vipera ammodytes ammodytes

Source Tissue

Source Tissue	Comment	Organism	Textmining
venom	five LAAO isozymes	Vipera ammodytes ammodytes	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
an L-amino acid + H2O + O2	L-amino-acid oxidase catalyzes the oxidative deamination of a number of L-amino acids to their respective imino acids with concomitant hydrolysis to alpha-keto acids, together with the production of ammonia and hydrogen peroxide	Vipera ammodytes ammodytes	a 2-oxo-acid + NH3 + H2O2	-	?

Subunits

Subunits	Comment	Organism
?	x * 55000, LAAO I, SDS-PAGE	Vipera ammodytes ammodytes
More	peptide mapping by mass spectrometry	Vipera ammodytes ammodytes

Synonyms

Synonyms	Comment	Organism
LAAO	-	Vipera ammodytes ammodytes

Cofactor

Cofactor	Comment	Organism	Structure
FAD	non-covalently bound	Vipera ammodytes ammodytes

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Release 2023.1 (January 2023)