Literature summary for 1.4.3.2 extracted from

Alves, R.M.; Antonucci, G.A.; Paiva, H.H.; Cintra, A.C.; Franco, J.J.; Mendonca-Franqueiro, E.P.; Dorta, D.J.; Giglio, J.R.; Rosa, J.C.; Fuly, A.L.; Dias-Baruffi, M.; Soares, A.M.; Sampaio, S.V.
Evidence of caspase-mediated apoptosis induced by l-amino acid oxidase isolated from Bothrops atrox snake venom (2008), Comp. Biochem. Physiol. A, 151, 542-550.

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Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	venom	Bothrops atrox	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
67000	-	x * 67000, SDS-PAGE	Bothrops atrox

Organism

Organism	UniProt	Comment	Textmining
Bothrops atrox	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Bothrops atrox

Purification (Commentary)

Purification (Comment)	Organism
native enzyme 54fold from venom by gel filtration and ultrafiltration, anion exchange chromatography, and lectin affinity chromatography to homogeneity	Bothrops atrox

Source Tissue

Source Tissue	Comment	Organism	Textmining
venom	-	Bothrops atrox	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
87.5	-	purified enzyme, substrate L-Leu	Bothrops atrox

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Ile + H2O + O2	-	Bothrops atrox	3-methyl-2-oxopentanoate + NH3 + H2O2	-	?
L-Leu + H2O + O2	-	Bothrops atrox	4-methyl-2-oxopentanoic acid + NH3 + H2O2	-	?
L-Met + H2O + O2	best substrate	Bothrops atrox	4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2	-	?
L-Phe + H2O + O2	-	Bothrops atrox	phenylpyruvate + NH3 + H2O2	-	?
L-Trp + H2O + O2	-	Bothrops atrox	indol-3-pyruvate + NH3 + H2O2	-	?
L-Tyr + H2O + O2	-	Bothrops atrox	4-hydroxyphenylpyruvate + NH3 + H2O2	-	?
additional information	substrate specificity, overview	Bothrops atrox	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 67000, SDS-PAGE	Bothrops atrox
More	peptide mapping	Bothrops atrox

Synonyms

Synonyms	Comment	Organism
LAAO	-	Bothrops atrox

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Bothrops atrox

pI Value

Organism	Comment	pI Value Maximum	pI Value
Bothrops atrox	isoelectric focussing	-	4.4

General Information

General Information	Comment	Organism
physiological function	the enzyme induces in vitro platelet aggregation, which may be due to H2O2 production, the addition of catalase completely inhibits the aggregation effect. It also shows cytotoxicity towards several cancer cell lines: HL60, Jurkat, B16F10 and PC12 causing apoptosis. The cytotoxicity activity is abolished by catalase	Bothrops atrox

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Release 2023.1 (January 2023)