Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.3.2 extracted from

  • Bregge-Silva, C.; Nonato, M.C.; de Albuquerque, S.; Ho, P.L.; Junqueira de Azevedo, I.L.; Vasconcelos Diniz, M.R.; Lomonte, B.; Rucavado, A.; Diaz, C.; Gutierrez, J.M.; Arantes, E.C.
    Isolation and biochemical, functional and structural characterization of a novel L-amino acid oxidase from Lachesis muta snake venom (2012), Toxicon, 60, 1263-1276.
    View publication on PubMed
Search for more literature for 1.4.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Lachesis muta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.97
-
 L-leucine at pH 8.0, temperature not specified in the publication Lachesis muta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56538
-
 2 * 56538, calculated from sequence of cDNA Lachesis muta
60000
-
 2 * 60000, SDS-PAGE Lachesis muta
60852
-
 2 * 60852, mass spectrometry Lachesis muta
120000
-
 gel filtration Lachesis muta

Organism

Organism UniProt Comment Textmining
Lachesis muta
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Mono Q column chromatography and Sephacryl S-100 gel filtration or phenyl Sepharose column chromatography, Affi-gel column chromatography, and Sephacryl S-200 gel filtration Lachesis muta

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Lachesis muta
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O + O2 about 10% activity compared to L-methionine Lachesis muta 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
L-glutamine + H2O + O2 about 5% activity compared to L-methionine Lachesis muta 2-oxoglutaramate + NH3 + H2O2
-
 ?
L-isoleucine + H2O + O2 about 35% activity compared to L-methionine Lachesis muta 3-methyl-2-oxopentanoate + NH3 + H2O2
-
 ?
L-leucine + H2O + O2 about 99% activity compared to L-methionine Lachesis muta 4-methyl-2-oxo-pentanoic acid + NH3 + H2O2
-
 ?
L-methionine + H2O + O2 100% activity Lachesis muta 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
L-phenylalanine + H2O + O2 about 55% activity compared to L-methionine Lachesis muta phenylpyruvate + NH3 + H2O2
-
 ?
L-tryptophan + H2O + O2 about 55% activity compared to L-methionine Lachesis muta indole-3-pyruvate + NH3 + H2O2
-
 ?
L-tyrosine + H2O + O2 about 40% activity compared to L-methionine Lachesis muta 4-hydroxyphenylpyruvate + NH3 + H2O2
-
 ?
L-valine + H2O + O2 less than 10% activity compared to L-methionine Lachesis muta 2-oxoisovalerate + NH3 + H2O2
-
 ?
additional information no activity with L-lysine, L-cysteine, L-serine, L-threonine, and L-cystine Lachesis muta ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 60000, SDS-PAGE Lachesis muta
homodimer 2 * 56538, calculated from sequence of cDNA Lachesis muta
homodimer 2 * 60852, mass spectrometry Lachesis muta

Synonyms

Synonyms Comment Organism
L-amino acid oxidase
-
 Lachesis muta
LAAO
-
 Lachesis muta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Lachesis muta

pH Range

pH Minimum pH Maximum Comment Organism
7 9 about 55% activity at pH 7.0, about 85% activity at pH 9.0 Lachesis muta

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Lachesis muta

pI Value

Organism Comment pI Value Maximum pI Value
Lachesis muta isoelectric focusing
-
 6.3

General Information

General Information Comment Organism
physiological function the enzyme induces cytotoxicity on AGS cell line (gastric adenocarcinoma) and on MCF-7 cell line (breast adenocarcinoma). It presents antiparasitic activity on Leishmania brasiliensis, but Trypanosoma cruzi is resistant to this enzyme Lachesis muta