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Literature summary for 1.4.3.2 extracted from

  • Zun, G.; Kos, J.; Sabotic, J.
    Higher fungi are a rich source of L-amino acid oxidases (2017), 3 Biotech, 7, 230 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.3.2

Application

Application Comment Organism
analysis in-gel detection of L-amino acid oxidase activity. L-amino acid concentrations in the range of 2-5 mM lead to activity bands of similar intensities, while higher concentrations results in partial substrate inhibition. Complete supplement mixture containing adenine, uracil, and L-amino acids (Arg, Asp, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Tyr, Val, each at different concentrations) is optimized at concentrations of 0.1-0.5% in combination with 1 and 2 mM o-phenylendiamine and horseradish peroxidase concentrations of 0.1-0.8 U/ml Basidiomycota sp.

Organism

Organism UniProt Comment Textmining
Basidiomycota sp.
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Synonyms

Synonyms Comment Organism
LAO
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 Basidiomycota sp.

General Information

General Information Comment Organism
additional information screening of L-amino acid oxidase activities in higher fungi using crude aqueous extracts of fruiting bodies of 22 basidiomycetes and 1 ascomycete. Half of these samples exhibit one to two bands of LAO activities with mostly broad substrate specificities and a variety of apparent molecular masses ranging from 25 to 200 kDa that are generally more active at pH 5.5 than at pH 8.0 Basidiomycota sp.