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Literature summary for 1.4.3.20 extracted from

  • Matsuda, M.; Asano, Y.
    Determination of plasma and serum L-lysine using L-lysine epsilon-oxidase from Marinomonas mediterranea NBRC 103028T (2010), Anal. Biochem., 406, 19-23.
    View publication on PubMed

Application

Application Comment Organism
medicine L-lysine epsilon-oxidase can be used for diagnosis based on plasma L-lysine concentration Marinomonas mediterranea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
L-lysine in 15 mM potassium phosphate buffer (pH 7.0), at 25┬░C Marinomonas mediterranea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
x * 97000, SDS-PAGE Marinomonas mediterranea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + O2 + H2O Marinomonas mediterranea 100% activity 2-aminoadipate 6-semialdehyde + H2O2 + NH3
-
?
L-lysine + O2 + H2O Marinomonas mediterranea NBRC 103028T 100% activity 2-aminoadipate 6-semialdehyde + H2O2 + NH3
-
?
L-ornithine + O2 + 2 H2O Marinomonas mediterranea 3.2% activity compared to L-lysine (2S)-2-hydroxy-5-oxopentanoate + 2 NH3 + H2O2
-
?
L-ornithine + O2 + 2 H2O Marinomonas mediterranea NBRC 103028T 3.2% activity compared to L-lysine (2S)-2-hydroxy-5-oxopentanoate + 2 NH3 + H2O2
-
?
additional information Marinomonas mediterranea no activity with L-phenylalanine, L-arginine, L-tyrosine, L-histidine, and L-leucine ?
-
?
additional information Marinomonas mediterranea NBRC 103028T no activity with L-phenylalanine, L-arginine, L-tyrosine, L-histidine, and L-leucine ?
-
?

Organism

Organism UniProt Comment Textmining
Marinomonas mediterranea
-
-
-
Marinomonas mediterranea NBRC 103028T
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Mono Q column chromatography Marinomonas mediterranea

Source Tissue

Source Tissue Comment Organism Textmining
additional information from blood plasma and serum Marinomonas mediterranea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
36.9
-
in 15 mM potassium phosphate buffer (pH 7.0), at 25┬░C Marinomonas mediterranea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + O2 + H2O 100% activity Marinomonas mediterranea 2-aminoadipate 6-semialdehyde + H2O2 + NH3
-
?
L-lysine + O2 + H2O 100% activity Marinomonas mediterranea NBRC 103028T 2-aminoadipate 6-semialdehyde + H2O2 + NH3
-
?
L-ornithine + O2 + 2 H2O 3.2% activity compared to L-lysine Marinomonas mediterranea (2S)-2-hydroxy-5-oxopentanoate + 2 NH3 + H2O2
-
?
L-ornithine + O2 + 2 H2O 3.2% activity compared to L-lysine Marinomonas mediterranea NBRC 103028T (2S)-2-hydroxy-5-oxopentanoate + 2 NH3 + H2O2
-
?
additional information no activity with L-phenylalanine, L-arginine, L-tyrosine, L-histidine, and L-leucine Marinomonas mediterranea ?
-
?
additional information no activity with L-phenylalanine, L-arginine, L-tyrosine, L-histidine, and L-leucine Marinomonas mediterranea NBRC 103028T ?
-
?

Subunits

Subunits Comment Organism
? x * 97000, SDS-PAGE Marinomonas mediterranea

Synonyms

Synonyms Comment Organism
L-lysine epsilon-oxidase
-
Marinomonas mediterranea

Cofactor

Cofactor Comment Organism Structure
lysyl-tyrosyl quinone
-
Marinomonas mediterranea