Cloned (Comment) | Organism |
---|---|
AOC2, cDNA library screening, DNA and amino acid sequence determination and analysis, transient expression of wild-type enzyme in HEK293, HEK293-EBNA or CHO cells | Homo sapiens |
AOC3, cDNA library screening, transient expression of wild-type and mutant enzymes in HEK293, HEK293-EBNA or CHO cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
M211V/Y394N/L469G | AOC3 mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Phenylethylamine | substrate inhibition | Homo sapiens | |
benzylamine | substrate inhibition | Homo sapiens | |
hydroxylamine | - |
Homo sapiens | |
Semicarbazide | - |
Homo sapiens | |
tryptamine | substrate inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | comparison of steady-state kinetics of enzyme expressed in CHO and HEK-293 EBNA cells, overview | Homo sapiens | |
0.056 | - |
tyramine | recombinant enzyme expressed in CHO cells | Homo sapiens | |
0.077 | - |
2-Phenylethylamine | recombinant enzyme expressed in CHO cells | Homo sapiens | |
0.167 | - |
benzylamine | recombinant enzyme expressed in CHO cells | Homo sapiens | |
0.178 | - |
tyramine | recombinant enzyme expressed in CHO cells | Homo sapiens | |
0.67 | - |
methylamine | recombinant enzyme expressed in CHO cells | Homo sapiens | |
1.7 | - |
methylamine | recombinant enzyme expressed in CHO cells | Homo sapiens | |
1.94 | - |
2-Phenylethylamine | recombinant enzyme expressed in CHO cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | - |
Homo sapiens | 9986 | - |
membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | dependent on | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylethylamine + H2O + O2 | Homo sapiens | - |
beta-phenylethanal + NH3 + H2O2 | - |
? | |
benzylamine + H2O + O2 | Homo sapiens | - |
benzaldehyde + NH3 + H2O2 | - |
? | |
benzylamine + H2O + O2 | Homo sapiens | low activity | benzaldehyde + NH3 + H2O2 | - |
? | |
methylamine + H2O + O2 | Homo sapiens | - |
formaldehyde + NH3 + H2O2 | - |
? | |
additional information | Homo sapiens | semicarbazide-sensitive amine oxidases constitute a group of copper-dependent enzymes, which oxidatively deaminate primary endo- and exogenous amines | ? | - |
? | |
tryptamine + H2O + O2 | Homo sapiens | - |
(1H-indol-3-yl)acetaldehyde + NH3 + H2O2 | - |
? | |
tyramine + H2O + O2 | Homo sapiens | - |
4-hydroxyphenylethanal + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O75106 | AOC2 | - |
Homo sapiens | Q16853 | AOC3 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
cartilage | - |
Homo sapiens | - |
eye | - |
Homo sapiens | - |
heart | - |
Homo sapiens | - |
kidney | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
lung | - |
Homo sapiens | - |
additional information | tissue expression pattern of AOC2, overview | Homo sapiens | - |
ovary | - |
Homo sapiens | - |
pancreas | - |
Homo sapiens | - |
prostate gland | - |
Homo sapiens | - |
retina | - |
Homo sapiens | - |
skin | - |
Homo sapiens | - |
testis | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylethylamine + H2O + O2 | - |
Homo sapiens | beta-phenylethanal + NH3 + H2O2 | - |
? | |
benzylamine + H2O + O2 | - |
Homo sapiens | benzaldehyde + NH3 + H2O2 | - |
? | |
benzylamine + H2O + O2 | low activity | Homo sapiens | benzaldehyde + NH3 + H2O2 | - |
? | |
methylamine + H2O + O2 | - |
Homo sapiens | formaldehyde + NH3 + H2O2 | - |
? | |
methylamine + H2O + O2 | - |
Homo sapiens | formaldehyde + NH3 + H2O2 | - |
r | |
additional information | semicarbazide-sensitive amine oxidases constitute a group of copper-dependent enzymes, which oxidatively deaminate primary endo- and exogenous amines | Homo sapiens | ? | - |
? | |
additional information | AOC2 is an enzymatically active cell surface SSAO with distinct substrate specificity, the preferred in vitro substrates of AOC2 are 2-phenylethylamine, tryptamine and p-tyramine, cf. EC 1.4.3.4, instead of methylamine and benzylamine, the favored substrates of AOC3. Substrate docking, molecular modeling and comparison of AOC2 and AOC3, overview. No activity of AOC2 with methylamine, polyamine spermidine, or histamine | Homo sapiens | ? | - |
? | |
additional information | substrate docking, molecular modeling and comparison of AOC2 and AOC3, overview. No activity of AOC3 with polyamine spermidine or histamine | Homo sapiens | ? | - |
? | |
tryptamine + H2O + O2 | - |
Homo sapiens | (1H-indol-3-yl)acetaldehyde + NH3 + H2O2 | - |
? | |
tryptamine + H2O + O2 | high activity | Homo sapiens | (1H-indol-3-yl)acetaldehyde + NH3 + H2O2 | - |
? | |
tyramine + H2O + O2 | - |
Homo sapiens | 4-hydroxyphenylethanal + NH3 + H2O2 | - |
? | |
tyramine + H2O + O2 | high activity | Homo sapiens | 4-hydroxyphenylethanal + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | AOC2 structure homology modelling, comparison with AOC3 | Homo sapiens |
More | AOC3 structure homology modelling, comparison with AOC2 | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
amine oxidase, copper containing | - |
Homo sapiens |
AOC2 | - |
Homo sapiens |
AOC3 | - |
Homo sapiens |
RAO | - |
Homo sapiens |
semicarbazide-sensitive amine oxidase | - |
Homo sapiens |
SSAO | - |
Homo sapiens |
VAP-1 | - |
Homo sapiens |
vascular adhesion protein-1 | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
tryptamine | recombinant enzyme expressed in CHO cells | Homo sapiens | |
0.08 | - |
2-Phenylethylamine | recombinant enzyme expressed in CHO cells | Homo sapiens | |
0.089 | - |
benzylamine | recombinant enzyme expressed in CHO cells | Homo sapiens |