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Literature summary for 1.4.3.21 extracted from

  • Kaitaniemi, S.; Elovaara, H.; Groen, K.; Kidron, H.; Liukkonen, J.; Salminen, T.; Salmi, M.; Jalkanen, S.; Elima, K.
    The unique substrate specificity of human AOC2, a semicarbazide-sensitive amine oxidase (2009), Cell. Mol. Life Sci., 66, 2743-2757.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
AOC2, cDNA library screening, DNA and amino acid sequence determination and analysis, transient expression of wild-type enzyme in HEK293, HEK293-EBNA or CHO cells Homo sapiens
AOC3, cDNA library screening, transient expression of wild-type and mutant enzymes in HEK293, HEK293-EBNA or CHO cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
M211V/Y394N/L469G AOC3 mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-Phenylethylamine substrate inhibition Homo sapiens
benzylamine substrate inhibition Homo sapiens
hydroxylamine
-
Homo sapiens
Semicarbazide
-
Homo sapiens
tryptamine substrate inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information comparison of steady-state kinetics of enzyme expressed in CHO and HEK-293 EBNA cells, overview Homo sapiens
0.056
-
tyramine recombinant enzyme expressed in CHO cells Homo sapiens
0.077
-
2-Phenylethylamine recombinant enzyme expressed in CHO cells Homo sapiens
0.167
-
benzylamine recombinant enzyme expressed in CHO cells Homo sapiens
0.178
-
tyramine recombinant enzyme expressed in CHO cells Homo sapiens
0.67
-
methylamine recombinant enzyme expressed in CHO cells Homo sapiens
1.7
-
methylamine recombinant enzyme expressed in CHO cells Homo sapiens
1.94
-
2-Phenylethylamine recombinant enzyme expressed in CHO cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
Homo sapiens 9986
-
membrane
-
Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ dependent on Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-phenylethylamine + H2O + O2 Homo sapiens
-
beta-phenylethanal + NH3 + H2O2
-
?
benzylamine + H2O + O2 Homo sapiens
-
benzaldehyde + NH3 + H2O2
-
?
benzylamine + H2O + O2 Homo sapiens low activity benzaldehyde + NH3 + H2O2
-
?
methylamine + H2O + O2 Homo sapiens
-
formaldehyde + NH3 + H2O2
-
?
additional information Homo sapiens semicarbazide-sensitive amine oxidases constitute a group of copper-dependent enzymes, which oxidatively deaminate primary endo- and exogenous amines ?
-
?
tryptamine + H2O + O2 Homo sapiens
-
(1H-indol-3-yl)acetaldehyde + NH3 + H2O2
-
?
tyramine + H2O + O2 Homo sapiens
-
4-hydroxyphenylethanal + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens O75106 AOC2
-
Homo sapiens Q16853 AOC3
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-
cartilage
-
Homo sapiens
-
eye
-
Homo sapiens
-
heart
-
Homo sapiens
-
kidney
-
Homo sapiens
-
liver
-
Homo sapiens
-
lung
-
Homo sapiens
-
additional information tissue expression pattern of AOC2, overview Homo sapiens
-
ovary
-
Homo sapiens
-
pancreas
-
Homo sapiens
-
prostate gland
-
Homo sapiens
-
retina
-
Homo sapiens
-
skin
-
Homo sapiens
-
testis
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phenylethylamine + H2O + O2
-
Homo sapiens beta-phenylethanal + NH3 + H2O2
-
?
benzylamine + H2O + O2
-
Homo sapiens benzaldehyde + NH3 + H2O2
-
?
benzylamine + H2O + O2 low activity Homo sapiens benzaldehyde + NH3 + H2O2
-
?
methylamine + H2O + O2
-
Homo sapiens formaldehyde + NH3 + H2O2
-
?
methylamine + H2O + O2
-
Homo sapiens formaldehyde + NH3 + H2O2
-
r
additional information semicarbazide-sensitive amine oxidases constitute a group of copper-dependent enzymes, which oxidatively deaminate primary endo- and exogenous amines Homo sapiens ?
-
?
additional information AOC2 is an enzymatically active cell surface SSAO with distinct substrate specificity, the preferred in vitro substrates of AOC2 are 2-phenylethylamine, tryptamine and p-tyramine, cf. EC 1.4.3.4, instead of methylamine and benzylamine, the favored substrates of AOC3. Substrate docking, molecular modeling and comparison of AOC2 and AOC3, overview. No activity of AOC2 with methylamine, polyamine spermidine, or histamine Homo sapiens ?
-
?
additional information substrate docking, molecular modeling and comparison of AOC2 and AOC3, overview. No activity of AOC3 with polyamine spermidine or histamine Homo sapiens ?
-
?
tryptamine + H2O + O2
-
Homo sapiens (1H-indol-3-yl)acetaldehyde + NH3 + H2O2
-
?
tryptamine + H2O + O2 high activity Homo sapiens (1H-indol-3-yl)acetaldehyde + NH3 + H2O2
-
?
tyramine + H2O + O2
-
Homo sapiens 4-hydroxyphenylethanal + NH3 + H2O2
-
?
tyramine + H2O + O2 high activity Homo sapiens 4-hydroxyphenylethanal + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
More AOC2 structure homology modelling, comparison with AOC3 Homo sapiens
More AOC3 structure homology modelling, comparison with AOC2 Homo sapiens

Synonyms

Synonyms Comment Organism
amine oxidase, copper containing
-
Homo sapiens
AOC2
-
Homo sapiens
AOC3
-
Homo sapiens
RAO
-
Homo sapiens
semicarbazide-sensitive amine oxidase
-
Homo sapiens
SSAO
-
Homo sapiens
VAP-1
-
Homo sapiens
vascular adhesion protein-1
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.032
-
tryptamine recombinant enzyme expressed in CHO cells Homo sapiens
0.08
-
2-Phenylethylamine recombinant enzyme expressed in CHO cells Homo sapiens
0.089
-
benzylamine recombinant enzyme expressed in CHO cells Homo sapiens