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Literature summary for 1.4.3.21 extracted from
- Implication for functions of the ectopic adipocyte copper amine oxidase (AOC3) from purified enzyme and cell-based kinetic studies (2012), PLoS ONE, 7, e29270.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Drosophila melanogaster Schneider-2 cells and in 3T3-L1 mouse adipocytes | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Semicarbazide | - |
Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.031 | - |
cysteamine | at pH 7.4, 37°C | Homo sapiens | |
| 0.066 | - |
aminoacetone | at pH 7.4, 37°C | Homo sapiens | |
| 0.0845 | - |
benzylamine | at pH 7.4, 37°C | Homo sapiens | |
| 0.099 | - |
dopamine | at pH 7.4, 37°C | Homo sapiens | |
| 0.652 | - |
methylamine | at pH 7.4, 37°C | Homo sapiens | |
| 2.05 | - |
phenethylamine | at pH 7.4, 37°C | Homo sapiens | |
| 2.65 | - |
Propylamine | at pH 7.4, 37°C | Homo sapiens | |
| 2.83 | - |
Butylamine | at pH 7.4, 37°C | Homo sapiens | |
| 3.42 | - |
Isobutylamine | at pH 7.4, 37°C | Homo sapiens | |
| 4.56 | - |
Isoamylamine | at pH 7.4, 37°C | Homo sapiens | |
| 5.71 | - |
amylamine | at pH 7.4, 37°C | Homo sapiens | |
| 12.8 | - |
ethylamine | at pH 7.4, 37°C | Homo sapiens | |
| 16.5 | - |
cyclohexanemethylamine | at pH 7.4, 37°C | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | required for activity, the zinc content is 0.4 mol per mole of AOC3 monomer | Homo sapiens | |
| Zn2+ | the zinc content is 0.16 mol per mole of AOC3 monomer | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q16853 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Sepharose column chromatography, and Sephacryl S-200 gel filtration | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipocyte | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aminoacetone + H2O + O2 | - |
Homo sapiens | methylglyoxal + NH3 + H2O2 | - |
? | |
| amylamine + H2O + O2 | - |
Homo sapiens | pentanal + NH3 + H2O2 | - |
? | |
| benzylamine + H2O + O2 | best substrate | Homo sapiens | benzaldehyde + NH3 + H2O2 | - |
? | |
| butylamine + H2O + O2 | - |
Homo sapiens | butanal + NH3 + H2O2 | - |
? | |
| cyclohexanemethylamine + H2O + O2 | - |
Homo sapiens | cyclohexanecarbaldehyde + NH3 + H2O2 | - |
? | |
| cysteamine + H2O + O2 | - |
Homo sapiens | sulfanylacetaldehyde + NH3 + H2O2 | - |
? | |
| dopamine + H2O + O2 | - |
Homo sapiens | ? | - |
? | |
| ethylamine + H2O + O2 | - |
Homo sapiens | acetaldehyde + NH3 + H2O2 | - |
? | |
| isoamylamine + H2O + O2 | - |
Homo sapiens | isoamylaldehyde + NH3 + H2O2 | - |
? | |
| isobutylamine + H2O + O2 | - |
Homo sapiens | isobutyraldehyde + NH3 + H2O2 | - |
? | |
| methylamine + H2O + O2 | - |
Homo sapiens | formaldehyde + NH3 + H2O2 | - |
? | |
| phenethylamine + H2O + O2 | - |
Homo sapiens | phenylacetaldehyde + NH3 + H2O2 | - |
? | |
| propylamine + H2O + O2 | - |
Homo sapiens | propanal + NH3 + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AOC3 | - |
Homo sapiens |
| Copper amine oxidase | - |
Homo sapiens |
| VAP-1 | - |
Homo sapiens |
| vascular adhesion protein-1 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.54 | - |
dopamine | at pH 7.4, 37°C | Homo sapiens | |
| 1.11 | - |
cysteamine | at pH 7.4, 37°C | Homo sapiens | |
| 1.12 | - |
phenethylamine | at pH 7.4, 37°C | Homo sapiens | |
| 1.46 | - |
aminoacetone | at pH 7.4, 37°C | Homo sapiens | |
| 3.42 | - |
benzylamine | at pH 7.4, 37°C | Homo sapiens | |
| 4.33 | - |
amylamine | at pH 7.4, 37°C | Homo sapiens | |
| 5.36 | - |
cyclohexanemethylamine | at pH 7.4, 37°C | Homo sapiens | |
| 5.6 | - |
methylamine | at pH 7.4, 37°C | Homo sapiens | |
| 5.8 | - |
Propylamine | at pH 7.4, 37°C | Homo sapiens | |
| 6.12 | - |
ethylamine | at pH 7.4, 37°C | Homo sapiens | |
| 6.31 | - |
Butylamine | at pH 7.4, 37°C | Homo sapiens | |
| 6.89 | - |
Isoamylamine | at pH 7.4, 37°C | Homo sapiens | |
| 7.4 | - |
Isobutylamine | at pH 7.4, 37°C | Homo sapiens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2,4,5-trihydroxyphenylalanine quinone | - |
Homo sapiens | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Homo sapiens | cells are induced to express the enzyme with 0.6 mM CuSO4 | up |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.325 | - |
cyclohexanemethylamine | at pH 7.4, 37°C | Homo sapiens | |
| 0.478 | - |
ethylamine | at pH 7.4, 37°C | Homo sapiens | |
| 0.545 | - |
phenethylamine | at pH 7.4, 37°C | Homo sapiens | |
| 0.757 | - |
amylamine | at pH 7.4, 37°C | Homo sapiens | |
| 1.51 | - |
Isoamylamine | at pH 7.4, 37°C | Homo sapiens | |
| 2.16 | - |
Isobutylamine | at pH 7.4, 37°C | Homo sapiens | |
| 2.19 | - |
Propylamine | at pH 7.4, 37°C | Homo sapiens | |
| 2.23 | - |
Butylamine | at pH 7.4, 37°C | Homo sapiens | |
| 5.44 | - |
dopamine | at pH 7.4, 37°C | Homo sapiens | |
| 8.58 | - |
methylamine | at pH 7.4, 37°C | Homo sapiens | |
| 22.3 | - |
aminoacetone | at pH 7.4, 37°C | Homo sapiens | |
| 35.5 | - |
cysteamine | at pH 7.4, 37°C | Homo sapiens | |
| 40.5 | - |
benzylamine | at pH 7.4, 37°C | Homo sapiens | |
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