Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 10 mM, 47% inhibition | Arthrobacter crystallopoietes | |
2-mercaptoethanol | 10 mM, 26% inhibition | Arthrobacter crystallopoietes | |
8-hydroxyquinoline | 10 mM, 24% inhibition | Arthrobacter crystallopoietes | |
alpha,alpha'-dipyridyl | 10 mM, 22% inhibition | Arthrobacter crystallopoietes | |
Diethyl dithiocarbamate | 10 mM, 76% inhibition | Arthrobacter crystallopoietes | |
dithiothreitol | 10 mM, 12% inhibition | Arthrobacter crystallopoietes | |
EDTA | 10 mM, 18% inhibition | Arthrobacter crystallopoietes | |
hydrazine | 10 mM, 95% inhibition | Arthrobacter crystallopoietes | |
Iproniazide | 10 mM, 43% inhibition | Arthrobacter crystallopoietes | |
isoniazide | 10 mM, 76% inhibition | Arthrobacter crystallopoietes | |
methylhydrazine | 10 mM, complete inhibition | Arthrobacter crystallopoietes | |
N,N-diethyldithiocarbamate | the inactive enzyme is restored with Cu2+ to 65% of its initial activity | Arthrobacter crystallopoietes | |
phenylhydrazine | 10 mM, complete inhibition | Arthrobacter crystallopoietes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.51 | - |
histamine | pH 7.0, 37°C | Arthrobacter crystallopoietes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | one atom of copper per mol of enzyme protein | Arthrobacter crystallopoietes | |
Cu2+ | a part of the apoenzyme is reconstituted in presence of Cu2+ | Arthrobacter crystallopoietes | |
MgSO4 | 10 mM, activation to 114% of control | Arthrobacter crystallopoietes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
81000 | - |
gel filtration, native PAGE | Arthrobacter crystallopoietes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter crystallopoietes | - |
KAIT-B-007 | - |
Arthrobacter crystallopoietes KAIT-B-007 | - |
KAIT-B-007 | - |
Purification (Comment) | Organism |
---|---|
- |
Arthrobacter crystallopoietes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.3 | - |
- |
Arthrobacter crystallopoietes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phenylethylamine + H2O + O2 | 44% of the activity with histamine | Arthrobacter crystallopoietes | 1-phenylethanal + NH3 + H2O2 | - |
? | |
1-phenylethylamine + H2O + O2 | 44% of the activity with histamine | Arthrobacter crystallopoietes KAIT-B-007 | 1-phenylethanal + NH3 + H2O2 | - |
? | |
agmatine + H2O + O2 | 4% of the activity with histamine | Arthrobacter crystallopoietes | ? | - |
? | |
agmatine + H2O + O2 | 4% of the activity with histamine | Arthrobacter crystallopoietes KAIT-B-007 | ? | - |
? | |
butylamine + H2O + O2 | 22% of the activity with histamine | Arthrobacter crystallopoietes | butanal + NH3 + H2O2 | - |
? | |
dopamine + H2O + O2 | 51% of the activity with histamine | Arthrobacter crystallopoietes | 2-(3,4-dihydroxyphenyl)ethanal + NH3 + H2O2 | - |
? | |
hexylamine + H2O + O2 | 41% of the activity with histamine | Arthrobacter crystallopoietes | hexanal + NH3 + H2O2 | - |
? | |
histamine + H2O + O2 | - |
Arthrobacter crystallopoietes | (imidazol-4-yl)acetaldehyde + NH3 + H2O2 | - |
? | |
histamine + H2O + O2 | - |
Arthrobacter crystallopoietes KAIT-B-007 | (imidazol-4-yl)acetaldehyde + NH3 + H2O2 | - |
? | |
additional information | no activity with putrescine, cadaverine, spermine, and spermidine | Arthrobacter crystallopoietes | ? | - |
? | |
additional information | no activity with putrescine, cadaverine, spermine, and spermidine | Arthrobacter crystallopoietes KAIT-B-007 | ? | - |
? | |
pentylamine + H2O + O2 | 32% of the activity with histamine | Arthrobacter crystallopoietes | pentanal + NH3 + H2O2 | - |
? | |
propylamine + H2O + O2 | 7% of the activity with histamine | Arthrobacter crystallopoietes | propanal + NH3 + H2O2 | - |
? | |
tryptamine + H2O + O2 | 15% of the activity with histamine | Arthrobacter crystallopoietes | 1H-indol-3-ylacetaldehyde + NH3 + H2O2 | - |
? | |
tyramine + H2O + O2 | 36% of the activity with histamine | Arthrobacter crystallopoietes | 4-hydroxyphenylethanal + NH3 + H2O2 | - |
? | |
tyramine + H2O + O2 | 36% of the activity with histamine | Arthrobacter crystallopoietes KAIT-B-007 | 4-hydroxyphenylethanal + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 81000, SDS-PAGE | Arthrobacter crystallopoietes |
Synonyms | Comment | Organism |
---|---|---|
histamine oxidase | - |
Arthrobacter crystallopoietes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
10 min, stable | Arthrobacter crystallopoietes |
70 | - |
10 min, 93% inactivation | Arthrobacter crystallopoietes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Arthrobacter crystallopoietes |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 37°C, 30 min, stable | Arthrobacter crystallopoietes |