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Literature summary for 1.4.3.22 extracted from

  • Sekiguchi, Y.; Makita, H.; Yamamura, A.; Matsumoto, K.
    A thermostable histamine oxidase from Arthrobacter crystallopoietes KAIT-B-007 (2004), J. Biosci. Bioeng., 97, 104-110.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 10 mM, 47% inhibition Arthrobacter crystallopoietes
2-mercaptoethanol 10 mM, 26% inhibition Arthrobacter crystallopoietes
8-hydroxyquinoline 10 mM, 24% inhibition Arthrobacter crystallopoietes
alpha,alpha'-dipyridyl 10 mM, 22% inhibition Arthrobacter crystallopoietes
Diethyl dithiocarbamate 10 mM, 76% inhibition Arthrobacter crystallopoietes
dithiothreitol 10 mM, 12% inhibition Arthrobacter crystallopoietes
EDTA 10 mM, 18% inhibition Arthrobacter crystallopoietes
hydrazine 10 mM, 95% inhibition Arthrobacter crystallopoietes
Iproniazide 10 mM, 43% inhibition Arthrobacter crystallopoietes
isoniazide 10 mM, 76% inhibition Arthrobacter crystallopoietes
methylhydrazine 10 mM, complete inhibition Arthrobacter crystallopoietes
N,N-diethyldithiocarbamate the inactive enzyme is restored with Cu2+ to 65% of its initial activity Arthrobacter crystallopoietes
phenylhydrazine 10 mM, complete inhibition Arthrobacter crystallopoietes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.51
-
histamine pH 7.0, 37°C Arthrobacter crystallopoietes

Metals/Ions

Metals/Ions Comment Organism Structure
copper one atom of copper per mol of enzyme protein Arthrobacter crystallopoietes
Cu2+ a part of the apoenzyme is reconstituted in presence of Cu2+ Arthrobacter crystallopoietes
MgSO4 10 mM, activation to 114% of control Arthrobacter crystallopoietes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81000
-
gel filtration, native PAGE Arthrobacter crystallopoietes

Organism

Organism UniProt Comment Textmining
Arthrobacter crystallopoietes
-
KAIT-B-007
-
Arthrobacter crystallopoietes KAIT-B-007
-
KAIT-B-007
-

Purification (Commentary)

Purification (Comment) Organism
-
Arthrobacter crystallopoietes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.3
-
-
Arthrobacter crystallopoietes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phenylethylamine + H2O + O2 44% of the activity with histamine Arthrobacter crystallopoietes 1-phenylethanal + NH3 + H2O2
-
?
1-phenylethylamine + H2O + O2 44% of the activity with histamine Arthrobacter crystallopoietes KAIT-B-007 1-phenylethanal + NH3 + H2O2
-
?
agmatine + H2O + O2 4% of the activity with histamine Arthrobacter crystallopoietes ?
-
?
agmatine + H2O + O2 4% of the activity with histamine Arthrobacter crystallopoietes KAIT-B-007 ?
-
?
butylamine + H2O + O2 22% of the activity with histamine Arthrobacter crystallopoietes butanal + NH3 + H2O2
-
?
dopamine + H2O + O2 51% of the activity with histamine Arthrobacter crystallopoietes 2-(3,4-dihydroxyphenyl)ethanal + NH3 + H2O2
-
?
hexylamine + H2O + O2 41% of the activity with histamine Arthrobacter crystallopoietes hexanal + NH3 + H2O2
-
?
histamine + H2O + O2
-
Arthrobacter crystallopoietes (imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
?
histamine + H2O + O2
-
Arthrobacter crystallopoietes KAIT-B-007 (imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
?
additional information no activity with putrescine, cadaverine, spermine, and spermidine Arthrobacter crystallopoietes ?
-
?
additional information no activity with putrescine, cadaverine, spermine, and spermidine Arthrobacter crystallopoietes KAIT-B-007 ?
-
?
pentylamine + H2O + O2 32% of the activity with histamine Arthrobacter crystallopoietes pentanal + NH3 + H2O2
-
?
propylamine + H2O + O2 7% of the activity with histamine Arthrobacter crystallopoietes propanal + NH3 + H2O2
-
?
tryptamine + H2O + O2 15% of the activity with histamine Arthrobacter crystallopoietes 1H-indol-3-ylacetaldehyde + NH3 + H2O2
-
?
tyramine + H2O + O2 36% of the activity with histamine Arthrobacter crystallopoietes 4-hydroxyphenylethanal + NH3 + H2O2
-
?
tyramine + H2O + O2 36% of the activity with histamine Arthrobacter crystallopoietes KAIT-B-007 4-hydroxyphenylethanal + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
monomer 1 * 81000, SDS-PAGE Arthrobacter crystallopoietes

Synonyms

Synonyms Comment Organism
histamine oxidase
-
Arthrobacter crystallopoietes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
10 min, stable Arthrobacter crystallopoietes
70
-
10 min, 93% inactivation Arthrobacter crystallopoietes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Arthrobacter crystallopoietes

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 37°C, 30 min, stable Arthrobacter crystallopoietes