KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00037 | - |
O2 | reaction performed with benzylamine. KM value is too low to be measurable. Therefore, it is calculated from KCAT_KM with putrescine | Pisum sativum | |
0.0446 | - |
O2 | reaction performed with putrescine | Pisum sativum | |
0.14 | - |
putrescine | - |
Pisum sativum | |
0.322 | - |
benzylamine | - |
Pisum sativum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | enzyme binds Cu. It is shown that PSAO exists predominantly in the Cu(I), topaquinone semiquinone state | Pisum sativum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | Q42432 | - |
- |
Purification (Comment) | Organism |
---|---|
isolated in 98% purity | Pisum sativum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
histamine + H2O + O2 = (imidazol-4-yl)acetaldehyde + NH3 + H2O2 | a mechanistic proposal for O2 reduction is advanced on the basis of thermodynamic considerations together with kinetic studies, the identification of steady-state intermediates, and the analysis of competitive oxygen kinetic isotope effects. The 18O kinetic isotope effect: 1.0136 at pH 7.2 is independent of temperature from 5°C to 47°C and insignificantly changed to 1.0122 upon raising the pH to 9, thus indicating the absence of kinetic complexity | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzylamine + H2O + O2 | - |
Pisum sativum | benzaldehyde + NH3 + H2O2 | - |
? | |
putrescine + H2O + O2 | - |
Pisum sativum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Pisum sativum |
Synonyms | Comment | Organism |
---|---|---|
Copper amine oxidase | - |
Pisum sativum |
pea seedling amine oxidase | - |
Pisum sativum |
PSAO | - |
Pisum sativum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Pisum sativum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.75 | - |
benzylamine | - |
Pisum sativum | |
91.5 | - |
putrescine | - |
Pisum sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Pisum sativum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2,4,5-trihydroxyphenylalanine quinone | it is shown that PSAO exists predominantly in the Cu(I), topaquinone semiquinone state | Pisum sativum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2100 | - |
O2 | determined with putrescine | Pisum sativum |