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Literature summary for 1.4.3.3 extracted from

  • Pollegioni, L.; Falbo, A.; Pilone, M.S.
    Specificity and kinetics of Rhodotorula gracilis D-amino acid oxidase (1992), Biochim. Biophys. Acta, 1120, 11-16.
    View publication on PubMed

General Stability

General Stability Organism
2-mercaptoethanol stabilizes Rhodotorula toruloides
changes in ionic strength, I: 16-82 mM, have only moderate effect on enzyme stability, stability maximum is 50 mM Rhodotorula toruloides
glycerol, 10%, stabilizes Rhodotorula toruloides

Inhibitors

Inhibitors Comment Organism Structure
1,3-Butadien 1-carboxylic acid D-phenylglycine oxidation Rhodotorula toruloides
2-hydroxybenzoic acid D-phenylglycine oxidation Rhodotorula toruloides
2-hydroxybutyric acid D-phenylglycine oxidation Rhodotorula toruloides
2-ketobutyric acid D-phenylglycine oxidation Rhodotorula toruloides
3-Bromobenzoic acid D-phenylglycine oxidation Rhodotorula toruloides
3-chlorobenzoic acid D-phenylglycine oxidation Rhodotorula toruloides
3-Hydroxybenzoic acid D-phenylglycine oxidation Rhodotorula toruloides
3-methylbenzoic acid D-phenylglycine oxidation Rhodotorula toruloides
3-Nitrobenzoic acid D-phenylglycine oxidation Rhodotorula toruloides
4-hydroxybenzoic acid D-phenylglycine oxidation Rhodotorula toruloides
benzoic acid D-phenylglycine oxidation Rhodotorula toruloides
crotonic acid D-phenylglycine oxidation Rhodotorula toruloides
kojic acid D-phenylglycine oxidation Rhodotorula toruloides
nicotinic acid D-phenylglycine oxidation Rhodotorula toruloides
Picolinic acid D-phenylglycine oxidation Rhodotorula toruloides
trans-2-Pentenoic acid D-phenylglycine oxidation Rhodotorula toruloides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
O2 with D-methionine Rhodotorula toruloides
0.29
-
D-phenylalanine
-
Rhodotorula toruloides
0.3
-
D-tryptophan
-
Rhodotorula toruloides
0.39
-
D-norleucine
-
Rhodotorula toruloides
0.5
-
D-leucine
-
Rhodotorula toruloides
0.83
-
D-methionine
-
Rhodotorula toruloides
0.89
-
D-Norvaline
-
Rhodotorula toruloides
13.75
-
D-serine
-
Rhodotorula toruloides
18.89
-
D-valine
-
Rhodotorula toruloides
21.41
-
D-cysteine
-
Rhodotorula toruloides
21.47
-
D-proline
-
Rhodotorula toruloides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-alanine + H2O + O2 Rhodotorula toruloides
-
pyruvic acid + NH3 + H2O2
-
?
D-cysteine + H2O + O2 Rhodotorula toruloides
-
2-oxo-3-thiopropionic acid + NH3 + H2O2
-
?
D-leucine + H2O + O2 Rhodotorula toruloides
-
4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
D-methionine + H2O + O2 Rhodotorula toruloides
-
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
?
D-norleucine + H2O + O2 Rhodotorula toruloides
-
2-oxohexanoate + NH3 + H2O2
-
?
D-norvaline + H2O + O2 Rhodotorula toruloides
-
2-oxopentanoate + NH3 + H2O2
-
?
D-phenylalanine + H2O + O2 Rhodotorula toruloides
-
phenylpyruvic acid + NH3 + H2O2
-
?
D-proline + H2O + O2 Rhodotorula toruloides
-
2-oxopentanoic acid + NH3 + H2O2
-
?
D-serine + H2O + O2 Rhodotorula toruloides
-
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
?
D-tryptophan + H2O + O2 Rhodotorula toruloides
-
indol-3-pyruvic acid + NH3 + H2O2
-
?
D-valine + H2O + O2 Rhodotorula toruloides
-
alpha-ketoisovaleric acid + NH3 + H2O2
-
?
thiazolidine-2-carboxylic acid + H2O + O2 Rhodotorula toruloides
-
? + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Rhodotorula toruloides
-
-
-

Reaction

Reaction Comment Organism Reaction ID
a D-amino acid + H2O + O2 = a 2-oxo carboxylate + NH3 + H2O2 ping pong bi bi mechanism Rhodotorula toruloides

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine + H2O + O2
-
Rhodotorula toruloides pyruvic acid + NH3 + H2O2
-
?
D-cysteine + H2O + O2
-
Rhodotorula toruloides 2-oxo-3-thiopropionic acid + NH3 + H2O2
-
?
D-leucine + H2O + O2
-
Rhodotorula toruloides 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
D-methionine + H2O + O2
-
Rhodotorula toruloides 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
?
D-norleucine + H2O + O2
-
Rhodotorula toruloides 2-oxohexanoate + NH3 + H2O2
-
?
D-norvaline + H2O + O2
-
Rhodotorula toruloides 2-oxopentanoate + NH3 + H2O2
-
?
D-phenylalanine + H2O + O2
-
Rhodotorula toruloides phenylpyruvic acid + NH3 + H2O2
-
?
D-proline + H2O + O2
-
Rhodotorula toruloides 2-oxopentanoic acid + NH3 + H2O2
-
?
D-serine + H2O + O2
-
Rhodotorula toruloides 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
?
D-tryptophan + H2O + O2
-
Rhodotorula toruloides indol-3-pyruvic acid + NH3 + H2O2
-
?
D-valine + H2O + O2
-
Rhodotorula toruloides alpha-ketoisovaleric acid + NH3 + H2O2
-
?
thiazolidine-2-carboxylic acid + H2O + O2
-
Rhodotorula toruloides ? + NH3 + H2O2
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
Rhodotorula toruloides

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 less than 50% of maximal activity above and below Rhodotorula toruloides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
523
-
D-valine
-
Rhodotorula toruloides
721
-
D-alanine
-
Rhodotorula toruloides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
Rhodotorula toruloides

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.3 7.9 30 min stable Rhodotorula toruloides

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Rhodotorula toruloides