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Literature summary for 1.4.3.3 extracted from
- Optimization of human D-amino acid oxidase expression in Escherichia coli (2009), Protein Expr. Purif., 68, 72-78.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional enzyme overexpression in Escherichia coli strain BL21(DE3), method optimization and upscaling, detailed overview | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| peroxisome | - |
Homo sapiens | 5777 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-alanine + H2O + O2 | Homo sapiens | - |
pyruvate + NH3 + H2O2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| flavoprotein | - |
Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3) | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8 | - |
purified recombinant enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-alanine + H2O + O2 | - |
Homo sapiens | pyruvate + NH3 + H2O2 | - |
? | |
| additional information | hDAAO exhibits optimal activity toward neutral D-amino acids and marginal activity toward basic ones, while acidic D-amino acids are not oxidized, structure-function relationship analysis, overview | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | structure-function relationship analysis, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DAAO | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | DAAO is a flavoprotein, the cofactor is loosely bound | Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | DAAO plays a key role in the pathophysiology of schizophrenia | Homo sapiens |
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Release 2023.1 (January 2023)
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