Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris strain CBS 7435, the enzyme production is enhanced to 350 kUnits/l of fed-batch bioreactor culture with a productivity of 3.1 kUnits/l h, subcloning in Escherichia coli. Expression optimzation and quantitative real-time PCR analysis expression analysis,overview | Rhodotorula toruloides |
Protein Variants | Comment | Organism |
---|---|---|
additional information | establishing the transformed Pichia pastoris, expressing RgDAO, as whole-cell catalyst | Rhodotorula toruloides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-methionine + H2O + O2 | Rhodotorula toruloides | - |
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula toruloides | P80324 | DAO1; i.e. Rhodosporidium toruloides | - |
Purification (Comment) | Organism |
---|---|
recombinant DAo from Pichia pastoris strain CBS 7435, the yeast cells are permeabilized by freeze-drying and incubation in 10% v/v 2-propanol resulting in a highly active, 1.6 kUnits/g dry matter, and stable oxidase preparation | Rhodotorula toruloides |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-methionine + H2O + O2 | - |
Rhodotorula toruloides | 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DAO | - |
Rhodotorula toruloides |