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Literature summary for 1.4.3.3 extracted from

  • Caldinelli, L.; Molla, G.; Bracci, L.; Lelli, B.; Pileri, S.; Cappelletti, P.; Sacchi, S.; Pollegioni, L.
    Effect of ligand binding on human D-amino acid oxidase: implications for the development of new drugs for schizophrenia treatment (2010), Protein Sci., 19, 1500-1512.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.3.3

Cloned(Commentary)

Cloned (Comment) Organism
expression of EGFP-tagged DAAO in U-87 glioblastoma cells, transient coexpression of pLG72 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
benzoate a classical substrate-competitive inhibitor, effect of benzoate on the FAD-binding process to hDAAO apoprotein Homo sapiens
chlorpromazine competitive versus cofactor FAD Homo sapiens
additional information benzoate and CPZ similarly modify the short-term cellular D-serine concentration but affect the cellular concentration of hDAAO differently Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information DAAO-lingand interaction kinetics, overview Homo sapiens
4
-
 D-serine pH 8.5, 25°C, recombinant EGFP-DAAO Homo sapiens
7
-
 D-serine pH 8.5, 25°C, recombinant wild-type DAAO Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-serine + H2O + O2 Homo sapiens the apoprotein form of hDAAO binds the substrate D-serine, which increases FAD binding thus increasing the amount of active holoenzyme in solution 2-oxo-3-hydroxypropionate + NH3 + H2O2
-
 ?
additional information Homo sapiens DAAO interacts with its physiological partner pLG72, the interaction is not inhibited by benzoate and chlorpromazine ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P14920
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-serine + H2O + O2
-
 Homo sapiens 2-oxo-3-hydroxypropionate + NH3 + H2O2
-
 ?
D-serine + H2O + O2 the apoprotein form of hDAAO binds the substrate D-serine, which increases FAD binding thus increasing the amount of active holoenzyme in solution Homo sapiens 2-oxo-3-hydroxypropionate + NH3 + H2O2
-
 ?
additional information DAAO interacts with its physiological partner pLG72, the interaction is not inhibited by benzoate and chlorpromazine Homo sapiens ?
-
 ?
additional information comparison of the kinetics of trypsin cleavage of hDAAO in the presence of various ligands, overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
DAAO
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5
-
 D-serine pH 8.5, 25°C, recombinant EGFP-DAAO Homo sapiens
3
-
 D-serine pH 8.5, 25°C, recombinant wild-type DAAO Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD the apoprotein form of hDAAO binds the substrate D-serine, this interaction increases FAD binding thus increasing the amount of active holoenzyme in solution, binding analysis of FAD, overview Homo sapiens

General Information

General Information Comment Organism
physiological function in human brain the flavoprotein D-amino acid oxidase is responsible for the degradation of the neuromodulator D-serine, an important effector of NMDA-receptor mediated neurotransmission Homo sapiens