Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzoate | moderate inhibition (49.7% residual activity at 1 mM) | Xenopus laevis | |
Ca2+ | 83.1% residual activity at 10 mM | Xenopus laevis | |
iodoacetamide | 89.8% residual activity at 1 mM | Xenopus laevis | |
iodoacetate | 81.6% residual activity at 1 mM | Xenopus laevis | |
malate | 78.6% residual activity at 10 mM | Xenopus laevis | |
Mg2+ | 84.9% residual activity at 10 mM | Xenopus laevis | |
p-chloromercuribenzoate | moderate inhibition (61.2% residual activity at 1 mM) | Xenopus laevis | |
p-hydroxymercuribenzoate | significant inhibition (6.2% residual activity at 1 mM) | Xenopus laevis | |
succinate | 90.7% residual activity at 1 mM | Xenopus laevis | |
Tartrate | 79.5% residual activity at 10 mM | Xenopus laevis | |
Zn2+ | 44% residual activity at 10 mM | Xenopus laevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 39000, SDS-PAGE | Xenopus laevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-amino acid + H2O + O2 | Xenopus laevis | - |
2-oxocarboxylate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenopus laevis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Toyopearl butyl column chromatography, and Q Sepharose column chromatography | Xenopus laevis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adult | - |
Xenopus laevis | - |
gut | - |
Xenopus laevis | - |
heart | lowest activity in heart | Xenopus laevis | - |
kidney | highest activity in kidney | Xenopus laevis | - |
larva | - |
Xenopus laevis | - |
liver | - |
Xenopus laevis | - |
lung | - |
Xenopus laevis | - |
stomach | - |
Xenopus laevis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0018 | - |
cell-free extract from tadpoles, with 20 mM D-proline in 20 mM Tris-HCl (pH 8.5) at 37°C | Xenopus laevis |
0.0135 | - |
cell-free extract from adults with 20 mM D-proline in 20 mM Tris-HCl (pH 8.5) at 37°C | Xenopus laevis |
0.165 | - |
enzyme from adults after 3563fold purification, with 20 mM D-proline in 20 mM Tris-HCl (pH 8.5) at 37°C | Xenopus laevis |
0.641 | - |
enzyme from tadpoles after 3563fold purification, with 20 mM D-proline in 20 mM Tris-HCl (pH 8.5) at 37°C | Xenopus laevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + H2O + O2 | 5.8% activity in tadpoles compared to D-proline | Xenopus laevis | pyruvate + NH3 + H2O2 | - |
? | |
D-amino acid + H2O + O2 | - |
Xenopus laevis | 2-oxocarboxylate + NH3 + H2O2 | - |
? | |
D-aspartate + H2O + O2 | 1.0% activity in tadpoles compared to D-proline | Xenopus laevis | oxaloacetate + NH3 + H2O2 | - |
? | |
D-leucine + H2O + O2 | 2.3% activity in tadpoles compared to D-proline | Xenopus laevis | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
D-methionine + H2O + O2 | 7.6% activity in tadpoles compared to D-proline | Xenopus laevis | 4-methylthio-2-oxobutanoic acid + NH3 + H2O2 | - |
? | |
D-phenylalanine + H2O + O2 | second best substrate | Xenopus laevis | phenylpyruvate + NH3 + H2O2 | - |
? | |
D-proline + H2O + O2 | 1.1% activity in tadpoles compared to D-proline | Xenopus laevis | 2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
D-proline + H2O + O2 | best substrate, 100% activity in tadpoles and adults | Xenopus laevis | 2-oxopentanoate + NH3 + H2O2 | - |
? | |
D-serine + H2O + O2 | 7.6% activity in tadpoles compared to D-proline | Xenopus laevis | 2-oxo-3-hydroxypropionic acid + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, SDS-PAGE | Xenopus laevis |
Synonyms | Comment | Organism |
---|---|---|
D-amino acid oxidase | - |
Xenopus laevis |
DAO | - |
Xenopus laevis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Xenopus laevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | 9 | - |
Xenopus laevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Xenopus laevis |
Organism | Comment | Expression |
---|---|---|
Xenopus laevis | enzyme activity is reduced to one-third in the adult | down |
Xenopus laevis | enzyme activity is increased until the final tadpole stage | up |
General Information | Comment | Organism |
---|---|---|
physiological function | D-amino acid oxidase functions in metamorphosis | Xenopus laevis |