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Literature summary for 1.4.3.3 extracted from
- Biochemical properties of human D-amino acid oxidase (2017), Front. Mol. Biosci., 4, 88 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-chlorobenzo[d]isoxazol-3-ol | a decrease in protein fluorescence is observed at increasing 6-chlorobenzo[d]isoxazol-3-ol concentrations | Homo sapiens |  |
| benzoate | a decrease in protein fluorescence is observed at increasing concentrations, with a biphasic change | Homo sapiens | |
| D-Dopa | - |
Homo sapiens | |
| additional information | not inhibitory: N-acetyl-cysteine at 10 mM. No change in protein fluorescence is observed with glycine (up to 50mM), ATP (up to 20mM), NMDA (up to 50mM), or D-glutamate (up to 50mM) | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
D-cysteine | pH 8.5, 25°C | Homo sapiens | |
| 0.7 | - |
D-kynurenine | pH 8.5, 25°C | Homo sapiens | |
| 6 | - |
D-Dopa | pH 8.5, 25°C | Homo sapiens | |
| 83.5 | - |
D-cycloserine | pH 8.5, 25°C | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | divalent ions and nucleotides do not affect enzyme function | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P14920 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-alanine + H2O + O2 | - |
Homo sapiens | pyruvate + H2O2 + NH3 | - |
? | |
| D-aspartate + H2O + O2 | - |
Homo sapiens | oxaloacetate + NH3 + H2O2 | - |
? | |
| D-cycloserine + H2O + O2 | - |
Homo sapiens | ? + NH3 + H2O2 | - |
? | |
| D-cysteine + H2O + O2 | - |
Homo sapiens | 2-oxo-3-sulfanylpropionate + NH3 + H2O2 | - |
? | |
| D-DOPA + H2O + O2 | - |
Homo sapiens | 3,4-dihydroxyphenylpyruvate + NH3 + H2O2 | - |
? | |
| D-glutamate + H2O + O2 | - |
Homo sapiens | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
| D-kynurenine + H2O + O2 | - |
Homo sapiens | kynurenate + NH3 + H2O2 | - |
? | |
| D-phenylalanine + H2O + O2 | - |
Homo sapiens | phenylpyruvate + NH3 + H2O2 | - |
? | |
| D-proline + H2O + O2 | - |
Homo sapiens | 2-oxopentanoate + NH3 + H2O2 | - |
? | |
| D-serine + H2O + O2 | - |
Homo sapiens | 2-oxo-3-hydroxypropanoate + NH3 + H2O2 | - |
? | |
| D-tyrosine + H2O + O2 | - |
Homo sapiens | 3-(4-hydroxyphenyl)pyruvate + NH3 + H2O2 | - |
? | |
| glycine + H2O + O2 | - |
Homo sapiens | glyoxylate + NH3 + H2O2 | - |
? | |
| additional information | DAAO is mainly active on neutral, hydrophobic and slightly polar D-amino acids and shows a preference for aromatic amino acids | Homo sapiens | ? | - |
- |
|
| N-methyl-D-aspartate + H2O + O2 | - |
Homo sapiens | oxaloacetate + methylamine + H2O2 | - |
? | |
| tryptophan + H2O + O2 | - |
Homo sapiens | indol-3-pyruvic acid + NH3 + H2O2 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
60 min pH 8.5, fully stable | Homo sapiens |
| 65 | - |
60 min, complete inactivation | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
D-kynurenine | pH 8.5, 25°C | Homo sapiens | |
| 0.77 | - |
D-cycloserine | pH 8.5, 25°C | Homo sapiens | |
| 8.6 | - |
D-cysteine | pH 8.5, 25°C | Homo sapiens | |
| 40.5 | - |
D-Dopa | pH 8.5, 25°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
substrate D-serine | Homo sapiens |
| 10 | - |
substrate D-alanine | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | 60 min, fully stable within | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | Kd value 0.008 mM for FAD-apoprotein complex. Benzoate binding favors a protein conformation with a higher affinity for the cofactor FAD | Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 41.3 | - |
D-Dopa | pH 8.5, 25°C | Homo sapiens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
D-cycloserine | pH 8.5, 25°C | Homo sapiens | |
| 0.14 | - |
D-kynurenine | pH 8.5, 25°C | Homo sapiens | |
| 6.75 | - |
D-Dopa | pH 8.5, 25°C | Homo sapiens | |
| 14.6 | - |
D-cysteine | pH 8.5, 25°C | Homo sapiens | |
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