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Literature summary for 1.4.3.4 extracted from

  • Vintem, A.P.; Price, N.T.; Silverman, R.B.; Ramsay, R.R.
    Mutation of surface cysteine 374 to alanine in monoamine oxidase A alters substrate turnover and inactivation by cyclopropylamines (2005), Bioorg. Med. Chem., 13, 3487-3495.
    View publication on PubMed
Search for more literature for 1.4.3.4

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type MAO A and mutant MAO A C374A in Pichia pastoris Homo sapiens

Protein Variants

Protein Variants Comment Organism
C374A mutation of surface cysteine 374 to alanine alters substrate turnover and inactivation by cyclopropylamines Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(N-cyclopropyl-alpha-methylbenzylamine) the inhibitor forms an adduct that allows reoxidation of the flavin on denaturation Homo sapiens
1-methyl-4-phenylpyridinium
-
 Homo sapiens
1-phenylcyclopropylamine the inhibitor forms an irreversible flavin adduct Homo sapiens
2-phenylcyclopropylamine the inhibitor forms an adduct that allows reoxidation of the flavin on denaturation Homo sapiens
D-amphetamine
-
 Homo sapiens
harman
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine pH 7.2, 30°C, wild-type enzyme Homo sapiens
0.039
-
 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
0.1
-
 kynuramine pH 7.2, 30°C, wild-type enzyme Homo sapiens
0.17
-
 kynuramine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
0.42
-
 benzylamine pH 7.2, 30°C, wild-type enzyme Homo sapiens
0.43
-
 serotonin pH 7.2, 30°C, wild-type enzyme Homo sapiens
0.44
-
 Phenylethylamine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
0.49
-
 Phenylethylamine pH 7.2, 30°C, wild-type enzyme Homo sapiens
0.51
-
 serotonin pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
0.61
-
 benzylamine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P21397
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine + H2O + O2
-
 Homo sapiens ?
-
 ?
benzylamine + H2O + O2
-
 Homo sapiens benzaldehyde + NH3 + H2O2
-
 ?
kynuramine + H2O + O2
-
 Homo sapiens 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2
-
 ?
phenylethylamine + H2O + O2
-
 Homo sapiens 2-phenylethanal + NH3 + H2O2
-
 ?
serotonin + H2O + O2
-
 Homo sapiens (5-hydroxy-1H-indol-3-yl)acetaldehyde + NH3 + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
MAO A
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.079
-
 benzylamine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
0.09
-
 benzylamine pH 7.2, 30°C, wild-type enzyme Homo sapiens
0.241
-
 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine pH 7.2, 30°C, wild-type enzyme Homo sapiens
0.29
-
 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
2.2
-
 kynuramine pH 7.2, 30°C, wild-type enzyme Homo sapiens
2.5
-
 Phenylethylamine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
2.5
-
 kynuramine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
3.1
-
 Phenylethylamine pH 7.2, 30°C, wild-type enzyme Homo sapiens
16
-
 serotonin pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
18.6
-
 serotonin pH 7.2, 30°C, wild-type enzyme Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000128
-
 harman pH 7.2, 30°C, wild-type enzyme Homo sapiens
0.00013
-
 harman pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
0.0035
-
 1-methyl-4-phenylpyridinium pH 7.2, 30°C, wild-type enzyme Homo sapiens
0.0045
-
 1-methyl-4-phenylpyridinium pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
0.0115
-
 D-amphetamine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
0.0144
-
 D-amphetamine pH 7.2, 30°C, wild-type enzyme Homo sapiens
0.2
-
 2-phenylcyclopropylamine pH 7.2, 30°C, wild-type enzyme Homo sapiens
0.31
-
 2-phenylcyclopropylamine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
1.4
-
 (N-cyclopropyl-alpha-methylbenzylamine) pH 7.2, 30°C, wild-type enzyme Homo sapiens
2.7
-
 (N-cyclopropyl-alpha-methylbenzylamine) pH 7.2, 30°C, mutant enzyme C374A Homo sapiens
7.3
-
 1-phenylcyclopropylamine pH 7.2, 30°C, wild-type enzyme Homo sapiens
12.8
-
 1-phenylcyclopropylamine pH 7.2, 30°C, mutant enzyme C374A Homo sapiens