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Literature summary for 1.4.3.5 extracted from

  • Merrill, A.H.; Horiike, K.; McCormick, D.B.
    Evidence for the regulation of pyridoxal 5-phosphate formation in liver by pyridoxamine (pyridoxine) 5 -phosphate oxidase (1978), Biochem. Biophys. Res. Commun., 83, 984-990.
    View publication on PubMed
Search for more literature for 1.4.3.5

Inhibitors

Inhibitors Comment Organism Structure
pyridoxal 5'-phosphate competitive inhibition; competitive product inhibition, overview Oryctolagus cuniculus
pyridoxal 5'-phosphate competitive inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 pyridoxine 5'-phosphate enzyme activity in liver homogenate Rattus norvegicus
0.008
-
 pyridoxamine 5'-phosphate enzyme activity in liver homogenate Rattus norvegicus
0.011
-
 pyridoxamine 5'-phosphate enzyme activity in liver homogenate Oryctolagus cuniculus
0.015
-
 pyridoxamine 5'-phosphate enzyme activity in cytosol Oryctolagus cuniculus
0.016
-
 pyridoxine 5'-phosphate pure enzyme in 200 mM potassium phosphate pH 7.0 Oryctolagus cuniculus
0.016
-
 pyridoxine 5'-phosphate pH 7.0, 37°C Oryctolagus cuniculus
0.017
-
 pyridoxine 5'-phosphate enzyme activity in cytosol Oryctolagus cuniculus
0.017
-
 pyridoxine 5'-phosphate enzyme activity in liver homogenate Oryctolagus cuniculus
0.018
-
 pyridoxamine 5'-phosphate pH 8.0, 37°C Oryctolagus cuniculus
0.022
-
 pyridoxine 5'-phosphate enzyme activity in liver homogenate in the presence of 0.005 mM pyridoxal 5'-phosphate Rattus norvegicus
0.024
-
 pyridoxamine 5'-phosphate pure enzyme in 200 mM potassium phosphate pH 7.0 Oryctolagus cuniculus
0.024
-
 pyridoxamine 5'-phosphate pH 7.0, 37°C Oryctolagus cuniculus
0.025
-
 pyridoxine 5'-phosphate pH 8.0, 37°C Oryctolagus cuniculus
0.039
-
 pyridoxamine 5'-phosphate enzyme activity in liver homogenate in the presence of 0.005 mM pyridoxal 5'-phosphate Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyridoxamine 5'-phosphate + H2O + O2 Oryctolagus cuniculus
-
 pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
pyridoxine 5'-phosphate + O2 Oryctolagus cuniculus
-
 pyridoxal 5'-phosphate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from liver Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-
liver
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridoxamine 5'-phosphate + H2O + O2
-
 Rattus norvegicus pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
pyridoxamine 5'-phosphate + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
pyridoxine 5'-phosphate + FMN
-
 Rattus norvegicus pyridoxal 5'-phosphate + FMNH2
-
 ?
pyridoxine 5'-phosphate + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
pyridoxamine (pyridoxine) 5'-phosphate oxidase
-
 Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 assay at Oryctolagus cuniculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.003
-
 pyridoxal 5'-phosphate pure enzyme in 200 mM potassium phosphate pH 7.0 or enzyme activity in liver homogenate, substrate pyridoxamine 5' phosphate or pyridoxine 5'-phosphate Oryctolagus cuniculus
0.003
-
 pyridoxal 5'-phosphate pH 8.0, 37°C Oryctolagus cuniculus