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Literature summary for 1.4.3.5 extracted from

  • Safo, M.K.; Musayev, F.N.; Schirch, V.
    Structure of Escherichia coli pyridoxine 5-phosphate oxidase in a tetragonal crystal form: insights into the mechanistic pathway of the enzyme (2005), Acta Crystallogr. Sect. D, 61, 599-604.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
5 mg/ml purified native enzyme with FMN bound in 100 mM K2PO4, pH 7.5, and 5 mM 2-mercaptoethanol, mixed with an equal volume of reservoir solution containing 60 mM HEPES, pH 6.8, and 0.65 M KH2PO4/NH4H2PO4, formation of tetragonal crystals within 4-5 weeks, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular-replacement method Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyridoxamine 5'-phosphate + FMN + H2O + 2 H+ Escherichia coli
-
pyridoxal 5'-phosphate + FMNH2 + NH3
-
?
pyridoxine 5'-phosphate + FMN Escherichia coli
-
pyridoxal 5'-phosphate + FMNH2
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AFI7
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme with tightly bound FMN Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2 reaction mechanism, the oxidation involves a stereospecific hydride-ion transfer between C4' of pyridoxal 5'-phosphate and N5 of FMN, structure-function relationship, substrate binding and active site structure Escherichia coli
pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2 reaction mechanism, the oxidation involves a stereospecific hydride-ion transfer between C4' of pyridoxal 5'-phosphate and N5 of FMN, structure-function relationship, substrate binding and active site structure Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridoxamine 5'-phosphate + FMN + H2O + 2 H+
-
Escherichia coli pyridoxal 5'-phosphate + FMNH2 + NH3
-
?
pyridoxine 5'-phosphate + FMN
-
Escherichia coli pyridoxal 5'-phosphate + FMNH2
-
?

Synonyms

Synonyms Comment Organism
ePNPOx
-
Escherichia coli
pyridoxine 5-phosphate oxidase
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FMN
-
Escherichia coli