Crystallization (Comment) | Organism |
---|---|
5 mg/ml purified native enzyme with FMN bound in 100 mM K2PO4, pH 7.5, and 5 mM 2-mercaptoethanol, mixed with an equal volume of reservoir solution containing 60 mM HEPES, pH 6.8, and 0.65 M KH2PO4/NH4H2PO4, formation of tetragonal crystals within 4-5 weeks, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular-replacement method | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxamine 5'-phosphate + FMN + H2O + 2 H+ | Escherichia coli | - |
pyridoxal 5'-phosphate + FMNH2 + NH3 | - |
? | |
pyridoxine 5'-phosphate + FMN | Escherichia coli | - |
pyridoxal 5'-phosphate + FMNH2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AFI7 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme with tightly bound FMN | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2 | reaction mechanism, the oxidation involves a stereospecific hydride-ion transfer between C4' of pyridoxal 5'-phosphate and N5 of FMN, structure-function relationship, substrate binding and active site structure | Escherichia coli | |
pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2 | reaction mechanism, the oxidation involves a stereospecific hydride-ion transfer between C4' of pyridoxal 5'-phosphate and N5 of FMN, structure-function relationship, substrate binding and active site structure | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxamine 5'-phosphate + FMN + H2O + 2 H+ | - |
Escherichia coli | pyridoxal 5'-phosphate + FMNH2 + NH3 | - |
? | |
pyridoxine 5'-phosphate + FMN | - |
Escherichia coli | pyridoxal 5'-phosphate + FMNH2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ePNPOx | - |
Escherichia coli |
pyridoxine 5-phosphate oxidase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Escherichia coli |