Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
pyridoxine 5'-phosphate | in 25 mM potassium phosphate, pH 7.8 and 25°C | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
2 * 27000, SDS-PAGE | Mycobacterium tuberculosis |
54000 | - |
gel filtration | Mycobacterium tuberculosis |
55380 | - |
nano-ESI mass spectroscopy | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mycobacterium tuberculosis | the enzyme does not recognize pyridoxamine 5'-phosphate as substrate | ? | - |
? | |
additional information | Mycobacterium tuberculosis H37Rv | the enzyme does not recognize pyridoxamine 5'-phosphate as substrate | ? | - |
? | |
pyridoxine 5'-phosphate + H2O + O2 | Mycobacterium tuberculosis | - |
pyridoxal 5'-phosphate + H2O2 | - |
? | |
pyridoxine 5'-phosphate + H2O + O2 | Mycobacterium tuberculosis H37Rv | - |
pyridoxal 5'-phosphate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme does not recognize pyridoxamine 5'-phosphate as substrate | Mycobacterium tuberculosis | ? | - |
? | |
additional information | the enzyme does not recognize pyridoxamine 5'-phosphate as substrate | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
pyridoxine 5'-phosphate + H2O + O2 | - |
Mycobacterium tuberculosis | pyridoxal 5'-phosphate + H2O2 | - |
? | |
pyridoxine 5'-phosphate + H2O + O2 | - |
Mycobacterium tuberculosis H37Rv | pyridoxal 5'-phosphate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 27000, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
PNPOx | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
pyridoxine 5'-phosphate | in 25 mM potassium phosphate, pH 7.8 and 25°C | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | contains one FMN molecule per dimer | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
pyridoxine 5'-phosphate | in 25 mM potassium phosphate, pH 7.8 and 25°C | Mycobacterium tuberculosis |