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Literature summary for 1.4.3.7 extracted from

  • Urich, K.
    D-Glutamtoxydase aus der Antennendruse des Flusskrebses Orconectes limosus: Reinigung und Charakterisierung (1968), Z. Naturforsch. B, 23, 1508-1511.
No PubMed abstract available

General Stability

General Stability Organism
very stable Faxonius limosus

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide 64% inhibition at 1 mM Faxonius limosus
L-aspartate slight inhibition at 100 mM Faxonius limosus
L-glutamate slight inhibition at 100 mM Faxonius limosus
p-chloromercuribenzoate 95% inhibition at 0.1 mM Faxonius limosus
succinate slight inhibition at 1 mM Faxonius limosus
Veronal strong inhibition Faxonius limosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4
-
D-Aspartate
-
Faxonius limosus
15.2
-
D-glutamate
-
Faxonius limosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-aspartate + H2O + O2 Faxonius limosus 20% higher activity with pure oxygen than in air oxaloacetate + NH3 + H2O2
-
?
D-glutamate + H2O + O2 Faxonius limosus highly specific, no deamination of other D-amino acids found, 30% higher activity with pure oxygen than in air 2-oxoglutarate + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Faxonius limosus
-
crayfish
-

Purification (Commentary)

Purification (Comment) Organism
partial Faxonius limosus

Source Tissue

Source Tissue Comment Organism Textmining
antennal gland
-
Faxonius limosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.23
-
-
Faxonius limosus

Storage Stability

Storage Stability Organism
-30┬░C, no loss of activity for several months Faxonius limosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-aspartate + H2O + O2 20% higher activity with pure oxygen than in air Faxonius limosus oxaloacetate + NH3 + H2O2
-
?
D-glutamate + H2O + O2 highly specific, no deamination of other D-amino acids found, 30% higher activity with pure oxygen than in air Faxonius limosus 2-oxoglutarate + NH3 + H2O2
-
?

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
21
-
stable for more than 5 h Faxonius limosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
with D-glutamate Faxonius limosus