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Literature summary for 1.4.7.1 extracted from

  • Ravasio, S.; Dossena, L.; Martin-Figueroa, E.; Florencio, F.J.; Mattevi, A.; Morandi, P.; Curti, B.; Vanoni, M.A.
    Properties of the recombinant ferredoxin-dependent glutamate synthase of Synechocystis PCC6803. Comparison with the Azospirillum brasilense NADPH-dependent enzyme and its isolated alpha subunit (2002), Biochemistry, 41, 8120-8133.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
6-diazo-5-oxo-norleucine reversible inactivator Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
 L-glutamate L-glutamate:iodonitrotetrazolium oxidoreductase activity at pH 9.5 Synechocystis sp.
0.1
-
 reduced iodonitrotetrazolium L-glutamate:iodonitrotetrazolium oxidoreductase activity at pH 9.5 Synechocystis sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Iron
-
 Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
-
-

Reaction

Reaction Comment Organism Reaction ID
2 L-glutamate + oxidized ferredoxin = L-glutamine + 2-oxoglutarate + reduced ferredoxin + 2 H+ proposed electron-transfer pathway Synechocystis sp.
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + oxidized iodonitrotetrazolium enzyme exhibits L-glutamate:iodonitrotetrazolium oxidoreductase activity at pH 7.5-9.5 Synechocystis sp. 2-oxoglutarate + NH3 + reduced iodonitrotetrazolium
-
 ?
L-glutamine + 2-oxoglutarate + reduced iodonitrotetrazolium + H+
-
 Synechocystis sp. L-glutamate + oxidized iodonitrotetrazolium
-
 r
L-glutamine + 2-oxoglutarate + reduced methylviologen significantly higher activity with ferredoxin than with methyl viologen Synechocystis sp. L-glutamate + oxidized methylviologen
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.67
-
 L-glutamate L-glutamate:iodonitrotetrazolium oxidoreductase activity at pH 9.5 Synechocystis sp.

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Synechocystis sp.