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Literature summary for 1.4.9.1 extracted from

  • Jones, L.H.; Pearson, A.R.; Tang, Y.; Wilmot, C.M.; Davidson, V.L.
    Active site aspartate residues are critical for tryptophan tryptophylquinone biogenesis in methylamine dehydrogenase (2005), J. Biol. Chem., 280, 17392-17396.
    View publication on PubMed
Search for more literature for 1.4.9.1

Protein Variants

Protein Variants Comment Organism
betaD32N preparation contains a major species with six disulfides but no oxygen incorporated into betaTrp57 and a minor species with both oxygens incorporated, which is active. 1000fold increase in KM-value for methylamine Paracoccus denitrificans
betaD76N mutant enzyme is completely inactive Paracoccus denitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 methylamine pH 8.5, 30°C, wild-type enzyme, acceptor amicyanin Paracoccus denitrificans
0.005
-
 methylamine pH 7.5, 30°C, wild-type enzyme, acceptor amicyanin Paracoccus denitrificans
0.015
-
 methylamine pH 7.5, 30°C, wild-type enzyme, acceptor phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans
0.021
-
 methylamine pH 8.5, 30°C, wild-type enzyme, acceptor phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans
12.2
-
 methylamine pH 8.5, 30°C, mutant enzyme betaD32N, acceptor amicyanin Paracoccus denitrificans
16.7
-
 methylamine pH 7.5, 30°C, mutant enzyme betaD32N, acceptor phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans
19.6
-
 methylamine pH 7.5, 30°C, mutant enzyme betaD32N, acceptor amicyanin Paracoccus denitrificans
22.4
-
 methylamine pH 8.5, 30°C, mutant enzyme betaD32N, acceptor phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methylamine + acceptor + H2O acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol or amicyanin Paracoccus denitrificans methanal + NH3 + reduced acceptor
-
 ?

Synonyms

Synonyms Comment Organism
MADH
-
 Paracoccus denitrificans
methylamine dehydrogenase
-
 Paracoccus denitrificans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.14
-
 methylamine pH 7.5, 30°C, mutant enzyme betaD32N, acceptor phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans
0.19
-
 methylamine pH 8.5, 30°C, mutant enzyme betaD32N, acceptor phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans
0.47
-
 methylamine pH 7.5, 30°C, mutant enzyme betaD32N, acceptor amicyanin Paracoccus denitrificans
1.2
-
 methylamine pH 8.5, 30°C, mutant enzyme betaD32N, acceptor amicyanin Paracoccus denitrificans
18
-
 methylamine pH 8.5, 30°C, wild-type enzyme, acceptor phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans
23
-
 methylamine pH 7.5, 30°C, wild-type enzyme, acceptor amicyanin Paracoccus denitrificans
26
-
 methylamine pH 8.5, 30°C, wild-type enzyme, acceptor amicyanin Paracoccus denitrificans
35
-
 methylamine pH 7.5, 30°C, wild-type enzyme, acceptor phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans