Literature summary for 1.4.99.6 extracted from

Ball, J.; Bui, Q.V.; Gannavaram, S.; Gadda, G.
Importance of glutamate 87 and the substrate alpha-amine for the reaction catalyzed by D-arginine dehydrogenase (2015), Arch. Biochem. Biophys., 568, 56-63.

Search for more literature for 1.4.99.6

Protein Variants

Protein Variants	Comment	Organism
E87L	kcat/Km pH-profile of the E87L mutant indicates only a single unprotonated group is required for maximal activity with D-arginine. E87 is the unprotonated group on the enzyme that binds cationic substrates	Pseudomonas aeruginosa
H48F	residue H48 is not responsible for the observed pKa value	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.8	-	D-leucine	wild-type, pH 6.0, 25°C	Pseudomonas aeruginosa
4.6	-	D-leucine	wild-type, pH 10.0, 25°C	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9HXE3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-arginine + phenazine methosulfate	-	Pseudomonas aeruginosa	5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate	overall reaction	?
D-leucine + phenazine methosulfate	-	Pseudomonas aeruginosa	? + NH3 + reduced phenazine methosulfate	-	?
D-lysine + phenazine methosulfate	-	Pseudomonas aeruginosa	6-amino-2-oxohexanoate + NH3 + reduced phenazine methosulfate	-	?
D-methionine + phenazine methosulfate	-	Pseudomonas aeruginosa	4-methylsulfanyl-2-oxobutanoate + NH3 + reduced phenazine methosulfate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.061	-	D-leucine	wild-type, pH 6.0, 25°C	Pseudomonas aeruginosa
55	-	D-leucine	wild-type, pH 10.0, 25°C	Pseudomonas aeruginosa
160	-	D-arginine	wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication	Pseudomonas aeruginosa
190	-	D-methionine	wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication	Pseudomonas aeruginosa
550	-	D-Lysine	wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication	Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.033	-	D-leucine	wild-type, pH 6.0, 25°C	Pseudomonas aeruginosa
12	-	D-leucine	wild-type, pH 10.0, 25°C	Pseudomonas aeruginosa
36	-	D-methionine	wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication	Pseudomonas aeruginosa
140	-	D-arginine	wild-type, pH-independent limiting value at high pH, pH not specified in the publication, 6°C	Pseudomonas aeruginosa
150	-	D-arginine	mutant E87L, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication	Pseudomonas aeruginosa
580	-	D-Lysine	wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication	Pseudomonas aeruginosa
1100	-	D-arginine	mutant H48F, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication	Pseudomonas aeruginosa
2600	-	D-arginine	wild-type, pH-independent limiting value at high pH, pH not specified in the publication, temperature not specified in the publication	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)