Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
A46G | mutation in loop L1. Probability of being in open conformation is higher than that of wild-type, yielding an increased level of solvent exposure of the active site. The flavin fluorescence intensity is about 2fold higher than in wild-type. Loop L1 is important for substrate capture and catalysis | Pseudomonas aeruginosa |
S45A | mutation in loop L1. Probability of being in open conformation is higher than that of wild-type, yielding an increased level of solvent exposure of the active site. The flavin fluorescence intensity is about 2fold higher than in wild-type. Loop L1 is important for substrate capture and catalysis | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HXE3 | - |
- |
Pseudomonas aeruginosa DSM 22644 | Q9HXE3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arginine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa | 5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate | overall reaction | ? | |
D-arginine + phenazine methosulfate + H2O | - |
Pseudomonas aeruginosa DSM 22644 | 5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate | overall reaction | ? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
D-arginine | mutant S45A, pH 5.5, 25°C | Pseudomonas aeruginosa | |
23 | - |
D-arginine | mutant A46G, pH 6.5, 25°C | Pseudomonas aeruginosa | |
210 | - |
D-arginine | mutant A46G, pH 10.0, 25°C | Pseudomonas aeruginosa | |
290 | - |
D-arginine | mutant S45A, pH 10.0, 25°C | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
D-arginine | mutant S45A, pH 5.5, 25°C | Pseudomonas aeruginosa | |
0.59 | - |
D-arginine | mutant A46G, pH 6.5, 25°C | Pseudomonas aeruginosa | |
270 | - |
D-arginine | mutant S45A, pH 10.0, 25°C | Pseudomonas aeruginosa | |
340 | - |
D-arginine | mutant A46G, pH 10.0, 25°C | Pseudomonas aeruginosa |