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Literature summary for 1.4.99.B3 extracted from

  • Panataleone, D.P.; Geller, A.M.; Taylor, P.P.
    Purification and characterization of an L-amino acid deaminase used to prepare unnatural amino acids (2001), J. Mol. Catal. B, 11, 795-803.
No PubMed abstract available
Search for more literature for 1.4.99.B3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Proteus myxofaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.28
-
 L-phenylalanine pH 7.5, 30°C Proteus myxofaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 x * 51000, SDS-PAGE Proteus myxofaciens

Organism

Organism UniProt Comment Textmining
Proteus myxofaciens A0A158RFS7
-
-
Proteus myxofaciens 4482 A0A158RFS7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + H2O + 2 cytochrome b
-
 Proteus myxofaciens 2-oxo-3-thiopropanoate + NH3 + 2 reduced cytochrome b 71.5% of the activiy with L-phenylalanine ?
L-cysteine + H2O + 2 cytochrome b
-
 Proteus myxofaciens 4482 2-oxo-3-thiopropanoate + NH3 + 2 reduced cytochrome b 71.5% of the activiy with L-phenylalanine ?
L-isoleucine + H2O + 2 cytochrome b
-
 Proteus myxofaciens 3-methyl-2-oxo-pentanoate + NH3 + 2 reduced cytochrome b 27.8% of the activiy with L-phenylalanine ?
L-isoleucine + H2O + 2 cytochrome b
-
 Proteus myxofaciens 4482 3-methyl-2-oxo-pentanoate + NH3 + 2 reduced cytochrome b 27.8% of the activiy with L-phenylalanine ?
L-leucine + H2O + 2 cytochrome b
-
 Proteus myxofaciens 2-oxo-4-methylpentanoate + NH3 + 2 reduced cytochrome b 111% of the activiy with L-phenylalanine ?
L-leucine + H2O + 2 cytochrome b
-
 Proteus myxofaciens 4482 2-oxo-4-methylpentanoate + NH3 + 2 reduced cytochrome b 111% of the activiy with L-phenylalanine ?
L-methionine + H2O + 2 cytochrome b
-
 Proteus myxofaciens 4-methylsulfanyl-2-oxobutanoate + NH3 + 2 reduced cytochrome b 102% of the activiy with L-phenylalanine ?
L-methionine + H2O + 2 cytochrome b
-
 Proteus myxofaciens 4482 4-methylsulfanyl-2-oxobutanoate + NH3 + 2 reduced cytochrome b 102% of the activiy with L-phenylalanine ?
L-phenylalanine + H2O + 2 cytochrome b
-
 Proteus myxofaciens phenylpyruvate + NH3 + 2 reduced cytochrome b
-
 ?
L-tryptophan + H2O + 2 cytochrome b
-
 Proteus myxofaciens 3-(1H-indol-3-yl)-2-oxopropanoic acid + NH3 + 2 reduced cytochrome b 90.6% of the activiy with L-phenylalanine ?
L-tyrosine + H2O + 2 cytochrome b
-
 Proteus myxofaciens 4-hydroxyphenylpyruvate + NH3 + 2 reduced cytochrome b 38.5% of the activiy with L-phenylalanine ?
additional information aliphatic, aromatic or sulfur-containing amino acids are preferred. The reaction has an absolute requirement for O2, releases NH3 and does not produce H2O2 Proteus myxofaciens ?
-
 ?
additional information aliphatic, aromatic or sulfur-containing amino acids are preferred. The reaction has an absolute requirement for O2, releases NH3 and does not produce H2O2 Proteus myxofaciens 4482 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 51000, SDS-PAGE Proteus myxofaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Proteus myxofaciens