Cloned (Comment) | Organism |
---|---|
gene lhpI, DNA and amino acid sequence determination and analysis, phylogenetic tree, genetic organization of genes encoding enzyme involved in the trans-3-hydroxy-L-proline pathway and networks with D-lysine and D-proline, overview. Recombinant expression of His6-tagged enzyme in Escherichia coli | Azospirillum brasilense |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a lphI knockout mutant, AbLhpI-, the mutant strain is distinct from the wild-type strain in that trans-3-hydroxy-L-proline, D-proline, and D-lysine do not support growth as a sole carbon source, but there is no difference in growth on other carbon sources, including trans-4-hydroxy-L-proline, between the two strains | Azospirillum brasilense |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.474 | - |
1-piperideine-2-carboxylate | purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C | Azospirillum brasilense | |
0.474 | - |
DELTA1-piperidine-2-carboxylate | pH 6.5, 30°C, coenzyme: NADPH | Azospirillum brasilense | |
0.491 | - |
1-pyrroline-(4R)-hydroxy-2-carboxylate | purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C | Azospirillum brasilense | |
0.491 | - |
DELTA1-pyrroline-(4R)-hydroxy-2-carboxylate | pH 6.5, 30°C, coenzyme: NADPH | Azospirillum brasilense | |
0.6 | - |
1-piperideine-2-carboxylate | purified recombinant His-tagged enzyme, with NADH, pH 6.5, 30°C | Azospirillum brasilense | |
0.6 | - |
DELTA1-piperidine-2-carboxylate | pH 6.5, 30°C, coenzyme: NADH | Azospirillum brasilense | |
0.628 | - |
1-pyrroline-2-carboxylate | purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C | Azospirillum brasilense | |
0.628 | - |
DELTA1-pyrroline-2-carboxylate | pH 6.5, 30°C, coenzyme: NADPH | Azospirillum brasilense | |
0.837 | - |
1-pyrroline-2-carboxylate | purified recombinant His-tagged enzyme, with NADH, pH 6.5, 30°C | Azospirillum brasilense | |
0.837 | - |
DELTA1-pyrroline-2-carboxylate | pH 6.5, 30°C, coenzyme: NADH | Azospirillum brasilense | |
2.1 | - |
(4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate | pH 6.5, 30°C, coenzyme: NADPH | Colwellia psychrerythraea | |
2.79 | - |
DELTA1-pyrroline-2-carboxylate | pH 6.5, 30°C, coenzyme: NADH | Colwellia psychrerythraea | |
3.63 | - |
L-proline | pH 10.5, 30°C, coenzyme: NADP+ | Azospirillum brasilense | |
3.99 | - |
L-proline | pH 10.5, 30°C, coenzyme: NAD+ | Azospirillum brasilense | |
5.9 | - |
DELTA1-pyrroline-2-carboxylate | pH 6.5, 30°C, coenzyme: NADPH | Colwellia psychrerythraea | |
6.54 | - |
L-pipecolate | pH 10.5, 30°C, coenzyme: NADP+ | Azospirillum brasilense | |
7.26 | - |
DELTA1-piperidine-2-carboxylate | pH 6.5, 30°C, coenzyme: NADH | Colwellia psychrerythraea | |
7.26 | - |
DELTA1-piperidine-2-carboxylate | pH 6.5, 30°C, coenzyme: NADPH | Colwellia psychrerythraea | |
14.8 | - |
L-pipecolate | pH 10.5, 30°C, coenzyme: NAD+ | Azospirillum brasilense | |
18.3 | - |
L-proline | purified recombinant His-tagged enzyme, with NADP+, pH 6.5, 30°C | Azospirillum brasilense | |
18.3 | - |
L-proline | pH 10.5, 30°C, coenzyme: NADP+ | Colwellia psychrerythraea | |
18.8 | - |
L-proline | purified recombinant His-tagged enzyme, with NAD+, pH 6.5, 30°C | Azospirillum brasilense | |
18.8 | - |
L-proline | pH 10.5, 30°C, coenzyme: NAD+ | Colwellia psychrerythraea | |
80 | - |
L-pipecolate | purified recombinant His-tagged enzyme, with NADP+, pH 6.5, 30°C | Azospirillum brasilense | |
80.1 | - |
L-pipecolate | pH 10.5, 30°C, coenzyme: NADP+ | Colwellia psychrerythraea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33312 | - |
calculated from sequence | Azospirillum brasilense |
36182 | - |
calculated from sequence | Colwellia psychrerythraea |
37000 | - |
SDS-PAGE | Azospirillum brasilense |
40000 | - |
SDS-PAGE | Colwellia psychrerythraea |
62000 | - |
recombinant enzyme, gel filtration | Azospirillum brasilense |
78000 | - |
gel filtration | Azospirillum brasilense |
81000 | - |
gel filtration | Colwellia psychrerythraea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-piperideine-2-carboxylate + NAD(P)H + H+ | Azospirillum brasilense | - |
L-pipecolate + NAD(P)+ | - |
r | |
1-pyrroline-2-carboxylate + NAD(P)H + H+ | Azospirillum brasilense | - |
L-proline + NAD(P)+ | - |
r | |
additional information | Azospirillum brasilense | trans-3-hydroxy-L-proline is converted to proline by the purified LhpH and LhpI proteins, i.e. trans-3-hydroxy-L-proline dehydratase and DELTA1-pyrroline-2-carboxylate reductase, at pH 7.0. LhpI has dual metabolic functions as a reductase for DELTA1-pyrroline-2-carboxylate and DELTA1-piperidine-2-carboxylate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azospirillum brasilense | V5YW53 | - |
- |
Colwellia psychrerythraea | Q485R8 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the organsim can grow on trans-3-hydroxy-L-proline and trans-4-hydroxy-L-proline as sole carbom sources, but also on D-proline and D-lysine | Azospirillum brasilense | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000457 | - |
substrates: L-pipecolate, NAD+, pH 10.5, 30°C | Colwellia psychrerythraea |
0.0005 | - |
purified recombinant His-tagged enzyme, substrates L-pipecolate and NAD+, pH 6.5, 30°C | Azospirillum brasilense |
0.00435 | - |
substrates: L-pipecolate, NADP+, pH 10.5, 30°C | Colwellia psychrerythraea |
0.0044 | - |
purified recombinant His-tagged enzyme, substrates L-pipecolate and NADP+, pH 6.5, 30°C | Azospirillum brasilense |
0.0045 | - |
purified recombinant His-tagged enzyme, substrates L-proline and NAD+, pH 6.5, 30°C | Azospirillum brasilense |
0.00451 | - |
substrates: L-proline, NAD+, pH 10.5, 30°C | Colwellia psychrerythraea |
0.0464 | - |
substrates: DELTA1-piperidine-2-carboxylate, NADH, pH 6.5, 30°C | Colwellia psychrerythraea |
0.174 | - |
substrates: (4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate, NADPH, pH 6.5, 30°C | Colwellia psychrerythraea |
0.291 | - |
substrates: DELTA1-piperidine-2-carboxylate, NADPH, pH 6.5, 30°C | Colwellia psychrerythraea |
0.63 | - |
purified recombinant His-tagged enzyme, substrates L-proline and NADP+, pH 6.5, 30°C | Azospirillum brasilense |
0.63 | - |
substrates: L-proline, NADP+, pH 10.5, 30°C | Colwellia psychrerythraea |
2.72 | - |
substrates: L-pipecolate, NAD+, pH 10.5, 30°C | Azospirillum brasilense |
2.92 | - |
substrates: L-pipecolate, NADP+, pH 10.5, 30°C | Azospirillum brasilense |
4.76 | - |
substrates: DELTA1-pyrroline-2-carboxylate, NADH, pH 6.5, 30°C | Colwellia psychrerythraea |
5.18 | - |
substrates: L-proline, NADP+, pH 10.5, 30°C | Azospirillum brasilense |
5.5 | - |
substrates: L-proline, NAD+, pH 10.5, 30°C | Azospirillum brasilense |
12 | - |
purified recombinant His-tagged enzyme, substrates 1-pyrroline-(4R)-hydroxy-2-carboxylate and NADH, pH 6.5, 30°C | Azospirillum brasilense |
12 | - |
substrates: DELTA1-pyrroline-(4R)-hydroxy-2-carboxylate, NADPH, pH 6.5, 30°C | Azospirillum brasilense |
30.2 | - |
substrates: DELTA1-pyrroline-2-carboxylate, NADPH, pH 6.5, 30°C | Colwellia psychrerythraea |
177 | - |
purified recombinant His-tagged enzyme, substrates 1-piperideine-2-carboxylate and NADH, pH 6.5, 30°C | Azospirillum brasilense |
177 | - |
substrates: DELTA1-piperidine-2-carboxylate, NADH, pH 6.5, 30°C | Azospirillum brasilense |
220 | - |
purified recombinant His-tagged enzyme, substrates 1-piperideine-2-carboxylate and NADPH, pH 6.5, 30°C | Azospirillum brasilense |
220 | - |
substrates: DELTA1-piperidine-2-carboxylate, NADPH, pH 6.5, 30°C | Azospirillum brasilense |
584 | - |
purified recombinant His-tagged enzyme, substrates 1-pyrroline-2-carboxylate and NADPH, pH 6.5, 30°C | Azospirillum brasilense |
584 | - |
substrates: DELTA1-pyrroline-2-carboxylate, NADPH, pH 6.5, 30°C | Azospirillum brasilense |
600 | - |
purified recombinant His-tagged enzyme, substrates 1-pyrroline-2-carboxylate and NADH, pH 6.5, 30°C | Azospirillum brasilense |
600 | - |
substrates: DELTA1-pyrroline-2-carboxylate, NADH, pH 6.5, 30°C | Azospirillum brasilense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate + NADPH + H+ | - |
Colwellia psychrerythraea | (4R)-4-hydroxy-L-proline + NADP+ | - |
r | |
1-piperideine-2-carboxylate + NAD(P)H + H+ | - |
Azospirillum brasilense | L-pipecolate + NAD(P)+ | - |
r | |
1-pyrroline-(4R)-hydroxy-2-carboxylate + NADPH + H+ | - |
Azospirillum brasilense | (4R)-hydroxy-L-proline + NADP+ | - |
r | |
1-pyrroline-2-carboxylate + NAD(P)H + H+ | - |
Azospirillum brasilense | L-proline + NAD(P)+ | - |
r | |
cis-3-hydroxy-L-proline + NADP+ | low activity | Azospirillum brasilense | ? + NADPH + H+ | - |
r | |
cis-4-hydroxy-L-proline + NADP+ | low activity | Azospirillum brasilense | ? + NADPH + H+ | - |
r | |
DELTA1-piperidine-2-carboxylate + NADH + H+ | - |
Azospirillum brasilense | L-pipecolate + NAD+ | - |
r | |
DELTA1-piperidine-2-carboxylate + NADH + H+ | - |
Colwellia psychrerythraea | L-pipecolate + NAD+ | - |
r | |
DELTA1-piperidine-2-carboxylate + NADPH + H+ | - |
Azospirillum brasilense | L-pipecolate + NADP+ | - |
r | |
DELTA1-piperidine-2-carboxylate + NADPH + H+ | - |
Colwellia psychrerythraea | L-pipecolate + NADP+ | - |
r | |
DELTA1-pyrroline-(4R)-hydroxy-2-carboxylate + NADPH + H+ | - |
Azospirillum brasilense | (4R)-hydroxy-L-proline + NADP+ | - |
r | |
DELTA1-pyrroline-2-carboxylate + NADH + H+ | - |
Azospirillum brasilense | L-proline + NAD+ | - |
r | |
DELTA1-pyrroline-2-carboxylate + NADH + H+ | - |
Colwellia psychrerythraea | L-proline + NAD+ | - |
r | |
DELTA1-pyrroline-2-carboxylate + NADPH + H+ | - |
Azospirillum brasilense | L-proline + NADP+ | - |
r | |
DELTA1-pyrroline-2-carboxylate + NADPH + H+ | - |
Colwellia psychrerythraea | L-proline + NADP+ | - |
r | |
L-pipecolate + NAD(P)+ | - |
Azospirillum brasilense | 1-piperideine-2-carboxylate + NAD(P)H + H+ | - |
r | |
L-pipecolate + NAD+ | - |
Azospirillum brasilense | DELTA1-piperidine-2-carboxylate + NADH + H+ | - |
r | |
L-pipecolate + NAD+ | - |
Colwellia psychrerythraea | DELTA1-piperidine-2-carboxylate + NADH + H+ | - |
r | |
L-pipecolate + NADP+ | - |
Azospirillum brasilense | DELTA1-piperidine-2-carboxylate + NADPH + H+ | - |
r | |
L-pipecolate + NADP+ | - |
Colwellia psychrerythraea | DELTA1-piperidine-2-carboxylate + NADPH + H+ | - |
r | |
L-proline + NAD(P)+ | - |
Azospirillum brasilense | 1-pyrroline-2-carboxylate + NAD(P)H + H+ | - |
r | |
L-proline + NAD+ | - |
Azospirillum brasilense | DELTA1-pyrroline-2-carboxylate + NADH + H+ | - |
r | |
L-proline + NAD+ | - |
Colwellia psychrerythraea | DELTA1-pyrroline-2-carboxylate + NADH + H+ | - |
r | |
L-proline + NADP+ | - |
Azospirillum brasilense | DELTA1-pyrroline-2-carboxylate + NADPH + H+ | - |
r | |
L-proline + NADP+ | - |
Colwellia psychrerythraea | DELTA1-pyrroline-2-carboxylate + NADPH + H+ | - |
r | |
additional information | trans-3-hydroxy-L-proline is converted to proline by the purified LhpH and LhpI proteins, i.e. trans-3-hydroxy-L-proline dehydratase and DELTA1-pyrroline-2-carboxylate reductase, at pH 7.0. LhpI has dual metabolic functions as a reductase for DELTA1-pyrroline-2-carboxylate and DELTA1-piperidine-2-carboxylate | Azospirillum brasilense | ? | - |
? | |
additional information | no activity with trans-4-hydroxy-D-proline, D-proline, trans-3-hydroxy-L-proline, cis-4-hydroxy-D-proline, and cis-3-hydroxy-D-proline, poor activity with trans-4-hydroxy-L-proline | Azospirillum brasilense | ? | - |
? | |
trans-4-hydroxy-L-proline + NADP+ | low activity | Azospirillum brasilense | ? + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Colwellia psychrerythraea |
dimer | 2 * 37000, SDS-PAGE | Azospirillum brasilense |
dimer | 2 * 33312, calculated from sequence | Azospirillum brasilense |
dimer | 2 * 36182, calculated from sequence | Colwellia psychrerythraea |
homodimer | 2 * 33313, sequence calculation, 2 * 37000, recombinant enzyme, SDS-PAGE | Azospirillum brasilense |
Synonyms | Comment | Organism |
---|---|---|
CphpI | - |
Colwellia psychrerythraea |
DELTA1-Pyr2C reductase | - |
Azospirillum brasilense |
DELTA1-Pyr2C reductase | - |
Colwellia psychrerythraea |
DELTA1-pyrroline-2-carboxylate reductase | - |
Azospirillum brasilense |
DELTA1-pyrroline-2-carboxylate reductase | - |
Colwellia psychrerythraea |
lhpI | - |
Azospirillum brasilense |
NAD(P)H-dependent DELTA1-pyrroline-2-carboxylate reductase | - |
Azospirillum brasilense |
Pyr2C reductase | - |
Azospirillum brasilense |
Pyr2C/Pip2C reductase | - |
Azospirillum brasilense |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Azospirillum brasilense |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0069 | - |
L-proline | purified recombinant His-tagged enzyme, with NAD+, pH 6.5, 30°C | Azospirillum brasilense | |
0.0069 | - |
L-proline | pH 10.5, 30°C, coenzyme: NAD+ | Colwellia psychrerythraea | |
0.02 | - |
L-pipecolate | pH 10.5, 30°C, coenzyme: NADP+ | Colwellia psychrerythraea | |
0.0213 | - |
L-pipecolate | purified recombinant His-tagged enzyme, with NADP+, pH 6.5, 30°C | Azospirillum brasilense | |
0.088 | - |
DELTA1-piperidine-2-carboxylate | pH 6.5, 30°C, coenzyme: NADH | Colwellia psychrerythraea | |
1.02 | - |
(4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate | pH 6.5, 30°C, coenzyme: NADPH | Colwellia psychrerythraea | |
1.09 | - |
L-proline | purified recombinant His-tagged enzyme, with NADP+, pH 6.5, 30°C | Azospirillum brasilense | |
1.09 | - |
L-proline | pH 10.5, 30°C, coenzyme: NADP+ | Colwellia psychrerythraea | |
1.38 | - |
DELTA1-piperidine-2-carboxylate | pH 6.5, 30°C, coenzyme: NADPH | Colwellia psychrerythraea | |
2.5 | - |
L-pipecolate | pH 10.5, 30°C, coenzyme: NADP+ | Azospirillum brasilense | |
3.7 | - |
L-pipecolate | pH 10.5, 30°C, coenzyme: NAD+ | Azospirillum brasilense | |
3.92 | - |
L-proline | pH 10.5, 30°C, coenzyme: NADP+ | Azospirillum brasilense | |
4.23 | - |
L-proline | pH 10.5, 30°C, coenzyme: NAD+ | Azospirillum brasilense | |
10.35 | - |
1-pyrroline-(4R)-hydroxy-2-carboxylate | purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C | Azospirillum brasilense | |
10.35 | - |
DELTA1-pyrroline-2-carboxylate | pH 6.5, 30°C, coenzyme: NADH | Colwellia psychrerythraea | |
10.35 | - |
DELTA1-pyrroline-(4R)-hydroxy-2-carboxylate | pH 6.5, 30°C, coenzyme: NADPH | Azospirillum brasilense | |
124.5 | - |
DELTA1-pyrroline-2-carboxylate | pH 6.5, 30°C, coenzyme: NADPH | Colwellia psychrerythraea | |
161.5 | - |
1-piperideine-2-carboxylate | purified recombinant His-tagged enzyme, with NADH, pH 6.5, 30°C | Azospirillum brasilense | |
161.5 | - |
DELTA1-piperidine-2-carboxylate | pH 6.5, 30°C, coenzyme: NADH | Azospirillum brasilense | |
165.8 | - |
DELTA1-piperidine-2-carboxylate | pH 6.5, 30°C, coenzyme: NADPH | Azospirillum brasilense | |
166 | - |
1-piperideine-2-carboxylate | purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C | Azospirillum brasilense | |
523 | - |
1-pyrroline-2-carboxylate | purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C | Azospirillum brasilense | |
523.3 | - |
DELTA1-pyrroline-2-carboxylate | pH 6.5, 30°C, coenzyme: NADPH | Azospirillum brasilense | |
615 | - |
1-pyrroline-2-carboxylate | purified recombinant His-tagged enzyme, with NADH, pH 6.5, 30°C | Azospirillum brasilense | |
615 | - |
DELTA1-pyrroline-2-carboxylate | pH 6.5, 30°C, coenzyme: NADH | Azospirillum brasilense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
reduction reaction | Azospirillum brasilense |
10.5 | - |
oxidation reaction | Azospirillum brasilense |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme shows dual specificity for NADPH and NADH | Azospirillum brasilense | |
NADH | - |
Azospirillum brasilense | |
NADPH | - |
Azospirillum brasilense |
Organism | Comment | Expression |
---|---|---|
Azospirillum brasilense | trans-3-hydroxy-L-proline dehydratase and NAD(P)H-dependent DELTA1-pyrroline-2-carboxylate reductase activity is induced by trans-3-hydroxy-L-proline, D-proline, and D-lysine, but not by trans-4-hydroxy-L-proline and cis-4-hydroxy-D-proline | up |
Azospirillum brasilense | induced by trans-3-hydroxy-L-proline (and D-proline and D-lysine) but not trans-4-hydroxy-L-proline | up |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme Pyr2C reductase is a member of the ornithine cyclodeaminase/micro-crystallin superfamily, different from known dpkA protein, and there are several significant differences in the enzymatic properties between Azospirillum brasilense and another bacteria: substrate and coenzyme specificities, phylogenetic analysis | Azospirillum brasilense |
malfunction | disruption of LhpI gene from Azospirillum brasilense leads to loss of growth on trans-3-hydroxy-L-proline (T3LHyp), D-proline and D-lysine | Azospirillum brasilense |
malfunction | disruption of LhpI gene from Azospirillum brasilense leads to loss of growth on trans-3-hydroxy-L-proline, D-proline and D-lysine, indicating that this gene has dual metabolic functions as a reductase for Pyr2C and DELTA1-piperidine-2-carboxylate in these pathways, and that the T3LHyp pathway is not linked to trans-4-hydroxy-L-proline and L-proline metabolism | Azospirillum brasilense |
metabolism | the enzyme is involved in the trans-3-hydroxy-L-proline pathway and the metabolic networks with D-lysine and D-proline, overview. The trans-3-hydroxy-L-proline pathway is not linked to trans-4-hydroxy-L-proline and L-proline metabolism | Azospirillum brasilense |
physiological function | enzyme AbLhpI is a bifunctional NAD(P)H-dependent Delta1-pyrroline-2-carboxylate/Delta1-piperideine-2-carboxylate reductase (Pyr2C/Pip2C reductase) | Azospirillum brasilense |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000027 | - |
L-pipecolate | pH 10.5, 30°C, coenzyme: NADP+ | Colwellia psychrerythraea | |
0.00027 | - |
L-pipecolate | purified recombinant His-tagged enzyme, with NADP+, pH 6.5, 30°C | Azospirillum brasilense | |
0.00037 | - |
L-proline | purified recombinant His-tagged enzyme, with NAD+, pH 6.5, 30°C | Azospirillum brasilense | |
0.00037 | - |
L-proline | pH 10.5, 30°C, coenzyme: NAD+ | Colwellia psychrerythraea | |
0.0418 | - |
DELTA1-piperidine-2-carboxylate | pH 6.5, 30°C, coenzyme: NADH | Colwellia psychrerythraea | |
0.0595 | - |
L-proline | pH 10.5, 30°C, coenzyme: NADP+ | Colwellia psychrerythraea | |
0.06 | - |
L-proline | purified recombinant His-tagged enzyme, with NADP+, pH 6.5, 30°C | Azospirillum brasilense | |
0.124 | - |
(4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate | pH 6.5, 30°C, coenzyme: NADPH | Colwellia psychrerythraea | |
0.19 | - |
DELTA1-piperidine-2-carboxylate | pH 6.5, 30°C, coenzyme: NADPH | Colwellia psychrerythraea | |
0.25 | - |
L-pipecolate | pH 10.5, 30°C, coenzyme: NAD+ | Azospirillum brasilense | |
0.38 | - |
L-pipecolate | pH 10.5, 30°C, coenzyme: NADP+ | Azospirillum brasilense | |
1.06 | - |
L-proline | pH 10.5, 30°C, coenzyme: NAD+ | Azospirillum brasilense | |
1.08 | - |
L-proline | pH 10.5, 30°C, coenzyme: NADP+ | Azospirillum brasilense | |
21.1 | - |
1-pyrroline-(4R)-hydroxy-2-carboxylate | purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C | Azospirillum brasilense | |
21.2 | - |
DELTA1-pyrroline-2-carboxylate | pH 6.5, 30°C, coenzyme: NADPH | Colwellia psychrerythraea | |
21.2 | - |
DELTA1-pyrroline-(4R)-hydroxy-2-carboxylate | pH 6.5, 30°C, coenzyme: NADPH | Azospirillum brasilense | |
225 | - |
DELTA1-pyrroline-2-carboxylate | pH 6.5, 30°C, coenzyme: NADH | Colwellia psychrerythraea | |
269 | - |
1-piperideine-2-carboxylate | purified recombinant His-tagged enzyme, with NADH, pH 6.5, 30°C | Azospirillum brasilense | |
270 | - |
DELTA1-piperidine-2-carboxylate | pH 6.5, 30°C, coenzyme: NADH | Azospirillum brasilense | |
350 | - |
1-piperideine-2-carboxylate | purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C | Azospirillum brasilense | |
351.7 | - |
DELTA1-piperidine-2-carboxylate | pH 6.5, 30°C, coenzyme: NADPH | Azospirillum brasilense | |
735 | - |
1-pyrroline-2-carboxylate | purified recombinant His-tagged enzyme, with NADH, pH 6.5, 30°C | Azospirillum brasilense | |
735 | - |
DELTA1-pyrroline-2-carboxylate | pH 6.5, 30°C, coenzyme: NADH | Azospirillum brasilense | |
833 | - |
1-pyrroline-2-carboxylate | purified recombinant His-tagged enzyme, with NADPH, pH 6.5, 30°C | Azospirillum brasilense | |
835 | - |
DELTA1-pyrroline-2-carboxylate | pH 6.5, 30°C, coenzyme: NADPH | Azospirillum brasilense |