Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.10 extracted from

  • Kingsbury, J.M.; Yang, Z.; Ganous, T.M.; Cox, G.M.; McCusker, J.H.
    Novel chimeric spermidine synthase-saccharopine dehydrogenase gene (SPE3-LYS9) in the human pathogen Cryptococcus neoformans (2004), Eukaryot. Cell, 3, 752-763.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.10

Cloned(Commentary)

Cloned (Comment) Organism
gene LYS9 is organized as a chimeric spermidine synthase-saccharopine dehydrogenase gene (SPE3-LYS9) encoding functional spermidine synthase, EC 2.5.1.16, and saccharopine dehydrogenase, DNA and amino acid sequence determination and analysis, expression of wild-type enzyme in Saccharomyces cerevisiae, the chimeric gene can complement a Saccharomyces cerevisiae lys9 mutant, but not a spe3 mutant Cryptococcus neoformans

Protein Variants

Protein Variants Comment Organism
additional information construction of 3 Spe3-Lys9 mutants, with defects in the spe3-part, the lys9-part, or both, which are auxotrophic for lysine and spermidine, spermidine, and lysine, respectively, transcription levels and phenotype overview, the polyamine auxotrophy due to defect spe3 cannot be overcome by spermine addition, while the mutan with lys 9 defect grows slowly at 30°C with lysine addition, but dies upon lysine starvation, the mutant with defects in both gene parts is avirulent and lethal Cryptococcus neoformans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O Cryptococcus neoformans enzyme catalyzes the penultimate step in lysine biosynthesis L-glutamate + 2-aminoadipate 6-semialdehyde + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Cryptococcus neoformans Q6RXX2 serotype A, strain H99, human pathogen, chimeric spermidine synthase-saccharopine dehydrogenase gene (SPE3-LYS9)
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 Cryptococcus neoformans L-glutamate + 2-aminoadipate 6-semialdehyde + NADPH + H+
-
 ?
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O enzyme catalyzes the penultimate step in lysine biosynthesis Cryptococcus neoformans L-glutamate + 2-aminoadipate 6-semialdehyde + NADPH + H+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Cryptococcus neoformans