Cloned (Comment) | Organism |
---|---|
gene LYS9 is organized as a chimeric spermidine synthase-saccharopine dehydrogenase gene (SPE3-LYS9) encoding functional spermidine synthase, EC 2.5.1.16, and saccharopine dehydrogenase, DNA and amino acid sequence determination and analysis, expression of wild-type enzyme in Saccharomyces cerevisiae, the chimeric gene can complement a Saccharomyces cerevisiae lys9 mutant, but not a spe3 mutant | Cryptococcus neoformans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of 3 Spe3-Lys9 mutants, with defects in the spe3-part, the lys9-part, or both, which are auxotrophic for lysine and spermidine, spermidine, and lysine, respectively, transcription levels and phenotype overview, the polyamine auxotrophy due to defect spe3 cannot be overcome by spermine addition, while the mutan with lys 9 defect grows slowly at 30°C with lysine addition, but dies upon lysine starvation, the mutant with defects in both gene parts is avirulent and lethal | Cryptococcus neoformans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | Cryptococcus neoformans | enzyme catalyzes the penultimate step in lysine biosynthesis | L-glutamate + 2-aminoadipate 6-semialdehyde + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cryptococcus neoformans | Q6RXX2 | serotype A, strain H99, human pathogen, chimeric spermidine synthase-saccharopine dehydrogenase gene (SPE3-LYS9) | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
Cryptococcus neoformans | L-glutamate + 2-aminoadipate 6-semialdehyde + NADPH + H+ | - |
? | |
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | enzyme catalyzes the penultimate step in lysine biosynthesis | Cryptococcus neoformans | L-glutamate + 2-aminoadipate 6-semialdehyde + NADPH + H+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Cryptococcus neoformans |