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Literature summary for 1.5.1.11 extracted from

  • Mueller, A.; Janssen, F.; Grieshaber, M.K.
    Putative reaction mechanism of heterologously expressed octopine dehydrogenase from the great scallop, Pecten maximus (L) (2007), FEBS J., 274, 6329-6339.
    View publication on PubMed
Search for more literature for 1.5.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli with a C-terminal penta His-tag Pecten maximus

Protein Variants

Protein Variants Comment Organism
C148A the mutant shows reduced activity Pecten maximus
C148S the mutant shows reduced activity and is only slightly inhibited by 5,5'-dithiobis(2-nitrobenzoic acid) and p-chloromercuribenzoate Pecten maximus
D329A the mutation results in strong increases in Km and decreases in kcat values for pyruvate and L-arginine, but has little effect on the Km and kcat values for NADH Pecten maximus
H212A the mutation results in strong increases in Km and decreases in kcat values for pyruvate and L-arginine, but has little effect on the Km and kcat values for NADH Pecten maximus
R324A the mutation results in strong increases in Km and decreases in kcat values for pyruvate and L-arginine, but has little effect on the Km and kcat values for NADH Pecten maximus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
 Pecten maximus
additional information ODH is not inhibited by 5 or 10 mM iodacetamide Pecten maximus
p-chloromercuribenzoate
-
 Pecten maximus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0198
-
 NADH wild type recombinant enzyme, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
0.0214
-
 NADH mutant enzyme D329A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
0.0389
-
 NADH mutant enzyme C148S, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
0.04
-
 NADH mutant enzyme C148A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
0.0649
-
 NADH mutant enzyme H212A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
0.0904
-
 NADH mutant enzyme R324A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
0.5
-
 L-Arg wild type recombinant enzyme, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
0.8
-
 pyruvate wild type recombinant enzyme, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
1.6
-
 L-Arg mutant enzyme C148A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
1.7
-
 L-Arg mutant enzyme C148S, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
2.5
-
 pyruvate mutant enzyme C148A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
2.6
-
 pyruvate mutant enzyme C148S, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
3.5
-
 L-Arg mutant enzyme H212A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
9.8
-
 L-Arg mutant enzyme D329A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
33
-
 pyruvate mutant enzyme D329A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
59
-
 pyruvate mutant enzyme H212A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
92
-
 pyruvate mutant enzyme R324A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
159
-
 L-Arg mutant enzyme R324A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 His-tagged recombinant enzyme, SDS-PAGE Pecten maximus
43300
-
 calculated from sequence of cDNA Pecten maximus

Organism

Organism UniProt Comment Textmining
Pecten maximus Q9BHM6
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity chromatography and Sephadex G-100 gel filtration Pecten maximus

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Pecten maximus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
63
-
 crude extract, at 18°C Pecten maximus
464
-
 His-tagged recombinant enzyme, after purification, at 18°C Pecten maximus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Arg + pyruvate + NADH
-
 Pecten maximus N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
N2-(D-1-carboxyethyl)-L-arginine:NAD+-oxidoreductase
-
 Pecten maximus
Octopine dehydrogenase
-
 Pecten maximus
ODH
-
 Pecten maximus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 L-Arg mutant enzyme D329A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
5.1
-
 L-Arg mutant enzyme H212A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
7.3
-
 NADH mutant enzyme H212A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
9.3
-
 NADH mutant enzyme D329A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
13
-
 pyruvate mutant enzyme H212A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
15.4
-
 pyruvate mutant enzyme D329A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
22.5
-
 pyruvate mutant enzyme R324A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
25
-
 NADH mutant enzyme R324A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
43
-
 L-Arg mutant enzyme R324A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
410
-
 NADH mutant enzyme C148S, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
422
-
 NADH mutant enzyme C148A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
444
-
 L-Arg mutant enzyme C148A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
446
-
 L-Arg mutant enzyme C148S, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
499
-
 pyruvate mutant enzyme C148A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
511
-
 pyruvate mutant enzyme C148S, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
640
-
 L-Arg wild type recombinant enzyme, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
652
-
 NADH wild type recombinant enzyme, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus
775
-
 pyruvate wild type recombinant enzyme, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C Pecten maximus

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Pecten maximus

pI Value

Organism Comment pI Value Maximum pI Value
Pecten maximus theoretical value
-
 5.35