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Literature summary for 1.5.1.15 extracted from

  • Mejia, N.; Rios-Orlandi, E.M.; Mackenzie, R.E.
    NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase from ascites tumor cells. Purification and properties (1986), J. Biol. Chem., 261, 9509-9513.
    View publication on PubMed
Search for more literature for 1.5.1.15

Activating Compound

Activating Compound Comment Organism Structure
arsenate 0.8 mM 104% activity Mus musculus
phosphate
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 L-5,10-methylenetetrahydrofolate
-
 Mus musculus
0.111
-
 NAD+
-
 Mus musculus
0.15
-
 5,10-methenyltetrahydrofolate
-
 Mus musculus
0.17
-
 phosphate
-
 Mus musculus
0.25
-
 Mg2+
-
 Mus musculus
0.793
-
 arsenate
-
 Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can substitute for Mg2+ Mus musculus
Mg2+
-
 Mus musculus
Mn2+
-
 Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 SDS-PAGE Mus musculus
68000
-
 cross-linking with bis(sulfosuccinimidyl)suberate Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-5,10-methenyl-tetrahydrofolate + NADH Mus musculus
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Clostridium formicaceticum
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Acetobacterium woodii
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Mus musculus
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Clostridium formicaceticum
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Acetobacterium woodii
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Acetobacterium woodii
-
-
-
Clostridium formicaceticum
-
-
-
Mus musculus
-
 mouse, Ehrlich ascites tumor cells
-

Purification (Commentary)

Purification (Comment) Organism
from ascites tumor cells Mus musculus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
38
-
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Mus musculus L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Clostridium formicaceticum L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Acetobacterium woodii L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Mus musculus 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Clostridium formicaceticum 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Acetobacterium woodii 5,10-methenyl-tetrahydrofolate + NADH
-
 r

Subunits

Subunits Comment Organism
dimer
-
 Clostridium formicaceticum
dimer
-
 Acetobacterium woodii
dimer 2 * 34000, native bifunctional enzyme, associated with cyclohydrolase, SDS-PAGE Mus musculus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Mus musculus
NAD+
-
 Clostridium formicaceticum
NAD+
-
 Acetobacterium woodii