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Literature summary for 1.5.1.15 extracted from

  • Barlowe, C.K.; Appling, D.R.
    Isolation and characterization of a novel eukaryotic monofunctional NAD+-dependent 5,10-methylenetetrahydrofolate dehydrogenase (1990), Biochemistry, 29, 7089-7094.
    View publication on PubMed
Search for more literature for 1.5.1.15

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 L-5,10-methylenetetrahydrofolate
-
 Saccharomyces cerevisiae
1.6
-
 NAD+
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-
cytoplasm
-
 Saccharomyces cerevisiae 5737
-
cytosol
-
 Homo sapiens 5829
-
cytosol
-
 Saccharomyces cerevisiae 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000 38000 SDS-PAGE Saccharomyces cerevisiae
64000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-5,10-methenyl-tetrahydrofolate + NADH Homo sapiens
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Rattus norvegicus
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Saccharomyces cerevisiae
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Clostridium formicaceticum
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Acetobacterium woodii
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH Saccharomyces cerevisiae KSY8
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Homo sapiens
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Rattus norvegicus
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Saccharomyces cerevisiae
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Clostridium formicaceticum
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Acetobacterium woodii
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+ Saccharomyces cerevisiae KSY8
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Acetobacterium woodii
-
-
-
Clostridium formicaceticum
-
-
-
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae
-
 yeast
-
Saccharomyces cerevisiae KSY8
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4
-
-
 Saccharomyces cerevisiae
27
-
-
 Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
4°C, enzyme activity is stable for several weeks after storage in the final elution buffer Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Homo sapiens L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Rattus norvegicus L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Saccharomyces cerevisiae L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Clostridium formicaceticum L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Acetobacterium woodii L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methenyl-tetrahydrofolate + NADH
-
 Saccharomyces cerevisiae KSY8 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Homo sapiens 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Rattus norvegicus 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Saccharomyces cerevisiae 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Clostridium formicaceticum 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Acetobacterium woodii 5,10-methenyl-tetrahydrofolate + NADH
-
 r
L-5,10-methylenetetrahydrofolate + NAD+
-
 Saccharomyces cerevisiae KSY8 5,10-methenyl-tetrahydrofolate + NADH
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 enzyme activity decreases approximately 2fold at pH 7.0 or pH 9.0 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
 Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 enzyme activity decreases approximately 2fold at pH 7.0 or pH 9.0 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens
NAD+
-
 Saccharomyces cerevisiae
NAD+
-
 Clostridium formicaceticum
NAD+
-
 Acetobacterium woodii