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Literature summary for 1.5.1.15 extracted from
- Mammalian fibroblasts lacking mitochondrial NAD+-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase are glycine auxotrophs (2003), J. Biol. Chem., 278, 19436-19441.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | primary fibroblasts from embryo of knock-out mice, spontaneous immortalization, the mutant cells require methionine and glycine for growth, glycine auxotrophy, replacement of mehtionine by homocysteine results in much lower enzyme activity, metabolism overview | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Mus musculus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Mus musculus |  |
| phosphate | required | Mus musculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + NAD+ | Mus musculus | - |
5,10-methenyltetrahydrofolate + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
bifunctional enzyme | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | enzyme is expressed during embryogenesis and in all immortilized cells | Mus musculus | - |
| fibroblast | primary embryonic | Mus musculus | - |
| additional information | cell growth studies, wild-type and mutant cell lines, overview | Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + NAD+ | - |
Mus musculus | 5,10-methenyltetrahydrofolate + NADH | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methylenetetrahydrofolate dehydrogenase-cyclohydrolase | - |
Mus musculus |
| methyleneTHF dehydrogenase | - |
Mus musculus |
| NMDMC | - |
Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | dependent on | Mus musculus | |
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Release 2023.1 (January 2023)
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