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Literature summary for 1.5.1.2 extracted from

  • Christensen, E.M.; Patel, S.M.; Korasick, D.A.; Campbell, A.C.; Krause, K.L.; Becker, D.F.; Tanner, J.J.
    Resolving the cofactor-binding site in the proline biosynthetic enzyme human pyrroline-5-carboxylate reductase 1 (2017), J. Biol. Chem., 292, 7233-7243.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
binary complexes of PYCR1 with NADPH or proline, at 1.9 A resolution, and a ternary complex containing NADPH and a P5C/proline analog, to 1.85 A resolution. NADPH is bound to the Rossmann fold. Structures provide a model of the Michaelis complex formed during hydride transfer Homo sapiens

Protein Variants

Protein Variants Comment Organism
T238A mutation in conserved residue, about 10fold decrease in catalytic efficiency Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 NADH wild-type, pH 7.5, 37°C Homo sapiens
0.159
-
 NADPH mutant T238A, pH 7.5, 37°C Homo sapiens
0.283
-
 NADPH wild-type, pH 7.5, 37°C Homo sapiens
0.667
-
 1-pyrroline-5-carboxylate cosubstrate NADPH, wild-type, pH 7.5, 37°C Homo sapiens
2.887
-
 1-pyrroline-5-carboxylate mutant T238A, wild-type, pH 7.5, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P32322
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pyrroline-5-carboxylate + NADH + H+
-
 Homo sapiens L-proline + NAD+
-
 ?
1-pyrroline-5-carboxylate + NADPH + H+
-
 Homo sapiens L-proline + NADP+
-
 ?

Subunits

Subunits Comment Organism
More enzyme forms a concentration-dependent decamer in solution, sedimentation velocity data Homo sapiens

Synonyms

Synonyms Comment Organism
Pycr1
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14
-
 1-pyrroline-5-carboxylate mutant T238A, wild-type, pH 7.5, 37°C Homo sapiens
23
-
 NADPH mutant T238A, pH 7.5, 37°C Homo sapiens
31
-
 1-pyrroline-5-carboxylate cosubstrate NADPH, wild-type, pH 7.5, 37°C Homo sapiens
74
-
 NADPH wild-type, pH 7.5, 37°C Homo sapiens
218
-
 NADH wild-type, pH 7.5, 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
50
-
 1-pyrroline-5-carboxylate mutant T238A, wild-type, pH 7.5, 37°C Homo sapiens
260
-
 NADPH wild-type, pH 7.5, 37°C Homo sapiens
470
-
 1-pyrroline-5-carboxylate cosubstrate NADPH, wild-type, pH 7.5, 37°C Homo sapiens
1400
-
 NADPH mutant T238A, pH 7.5, 37°C Homo sapiens
3100
-
 NADH wild-type, pH 7.5, 37°C Homo sapiens