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Literature summary for 1.5.1.2 extracted from
- delta1-Pyrroline-5-carboxylate reductase from Arabidopsis thaliana: stimulation or inhibition by chloride ions and feedback regulation by proline depend on whether NADPH or NADH acts as co-substrate (2014), New Phytol., 202, 911-919.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Esherichia coli | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCl | inhibitory on NADH-dependent reaction | Arabidopsis thaliana |  |
| additional information | not inhibitory: NAD+ | Arabidopsis thaliana | |
| NaCl | inhibitory on NADH-dependent reaction | Arabidopsis thaliana | |
| NADP+ | - |
Arabidopsis thaliana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.026 | - |
NADPH | pH 7.8, 35°C | Arabidopsis thaliana | |
| 0.096 | - |
1-pyrroline-5-carboxylate | cofactor NADPH, pH 7.8, 35°C | Arabidopsis thaliana | |
| 0.21 | - |
NADH | pH 7.8, 35°C | Arabidopsis thaliana | |
| 0.915 | - |
1-pyrroline-5-carboxylate | cofactor NADH, pH 7.8, 35°C | Arabidopsis thaliana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | stimulation of NADPH-dependent reaction, 70% stimulation at 100 mM | Arabidopsis thaliana | |
| NaCl | stimulation of NADPH-dependent reaction, 70% stimulation at 100 mM | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
- |
Arabidopsis thaliana |
| 354000 | - |
gel filtration | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | F4K884 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| callus | cell suspension culture established from cotyledon-derived calli | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-pyrroline-5-carboxylate + NADH + H+ | - |
Arabidopsis thaliana | L-proline + NAD+ | - |
? | |
| 1-pyrroline-5-carboxylate + NADPH + H+ | - |
Arabidopsis thaliana | L-proline + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dodecamer | 12 * 28000, SDS-PAGE | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 694 | - |
NADPH | pH 7.8, 35°C | Arabidopsis thaliana | |
| 3049 | - |
NADH | pH 7.8, 35°C | Arabidopsis thaliana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
cofactor NADH | Arabidopsis thaliana |
| 8.4 | - |
cofactor NADPH | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Arabidopsis thaliana | |
| NADPH | - |
Arabidopsis thaliana | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Arabidopsis thaliana | isoelectric focusing | - |
4.7 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.032 | - |
cofactor NADH, pH 7.8, 35°C | Arabidopsis thaliana | NADP+ | |
| 1.44 | - |
cofactor NADPH, pH 7.8, 35°C | Arabidopsis thaliana | NADP+ | |
| 200 | - |
cofactor NADH, pH 7.8, 35°C | Arabidopsis thaliana | NaCl | |
| 200 | - |
cofactor NADH, pH 7.8, 35°C | Arabidopsis thaliana | KCl | |
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Release 2023.1 (January 2023)
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