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Literature summary for 1.5.1.20 extracted from

  • Matthews, R.G.
    Methylenetetrahydrofolate reductase from pig liver (1986), Methods Enzymol., 122, 372-381.
    View publication on PubMed

General Stability

General Stability Organism
extremely sensitive to proteolysis Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
dihydrofolate
-
Sus scrofa
dihydropteroylhexaglutamate
-
Sus scrofa
dihydropteroylpolyglutamate most potent inhibitor is dihydropteroylhexaglutamate Sus scrofa
S-adenosylmethionine inhibition partially reversed by S-adenosylhomocysteine Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
NADPH 5,10-methylenetetrahydrofolate reductase reaction, pH 7.2 Sus scrofa
0.019
-
5,10-methylenetetrahydrofolate methylenetetrahydrofolate reductase reaction, pH 6.7 Sus scrofa
0.088
-
5,10-methylenetetrahydrofolate methylenetetrahydrofolate reductase reaction, pH 7.2 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77300
-
SDS-PAGE Sus scrofa
136000
-
scanning transmission electron microscopy Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + NADPH Sus scrofa initial enzyme in pathway leading to synthesis of S-adenosylmethionine ?
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H + H+ mechanism Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
different assay methods Sus scrofa
19.4
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
-70°C, 10% glycerol, several months Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pyrroline-5-carboxylate + NADH + H+
-
Sus scrofa L-proline + NAD+
-
r
5,10-methylenetetrahydrofolate + NADPH ping pong kinetics Sus scrofa 5-methyltetrahydrofolate + NADP+
-
?
5,10-methylenetetrahydrofolate + NADPH enzyme also catalyzes the NADPH-linked reduction of quinoid dihydrofolate and dihydropterin derivates Sus scrofa 5-methyltetrahydrofolate + NADP+
-
?
5,10-methylenetetrahydrofolate + NADPH initial enzyme in pathway leading to synthesis of S-adenosylmethionine Sus scrofa ?
-
?
5-methyltetrahydrofolate + oxidized menadione ping pong kinetics Sus scrofa 5,10-methylenetetrahydrofolate + reduced menadione
-
?
NADPH + H+ + menadione ping pong kinetics Sus scrofa NADP+ + menadiol
-
?

Subunits

Subunits Comment Organism
dimer dimer or trimer, 2 or 3 * 77300, SDS-PAGE Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
160
-
NADH
-
Sus scrofa

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Sus scrofa
FAD each subunit contains noncovalently bound FAD Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000013
-
dihydropteroylhexaglutamate
-
Sus scrofa