Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(+)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate | inhibits at high concentrations | Rattus norvegicus | |
S-adenosylmethionine | in vivo feedback-inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
(+)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate | - |
Rattus norvegicus | |
0.023 | - |
5-methyltetrahydrofolate | - |
Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
adult male Sprague-Dawley rats, fed a Purina lab chow diet | - |
Purification (Comment) | Organism |
---|---|
more than 100fold partial purification | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity is greater with the monoglutamate substrate than with the pentaglutamate | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate + reduced acceptor | equilibrium lies far in favor of 5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate formation, pentaglutamate form binds to the same enzyme site as monoglutamate form | Rattus norvegicus | (+)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate + oxidized acceptor | menadione as acceptor for 5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate formation | ? | |
5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: reduced acceptor is FADH2 | Rattus norvegicus | 5-methyltetrahydrofolate + oxidized acceptor | product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate | ? | |
5,10-methylenetetrahydrofolate + reduced acceptor | 5,10-methylene-5,6,7,8-tetrahydropteroylmonoglutamate as substrate | Rattus norvegicus | 5-methyltetrahydrofolate + oxidized acceptor | product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate | ? | |
5,10-methylenetetrahydrofolate + reduced acceptor | equilibrium lies far in favor of 5-methyltetrahydrofolate formation | Rattus norvegicus | 5-methyltetrahydrofolate + oxidized acceptor | product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate | ? | |
5,10-methylenetetrahydrofolate + reduced acceptor | reverse reaction: menadione as electron acceptor | Rattus norvegicus | 5-methyltetrahydrofolate + oxidized acceptor | product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate | ? | |
5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: NADH as reduced acceptor | Rattus norvegicus | 5-methyltetrahydrofolate + oxidized acceptor | product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate | ? | |
5-methyltetrahydrofolate + NAD+ | - |
Rattus norvegicus | 5,10-methylenetetrahydrofolate + NADH + H+ | - |
r | |
additional information | reverse reaction: not NADP+ as acceptor | Rattus norvegicus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
5-methyltetrahydrofolate and 5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate as substrates | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | pH 6: about 70% of activity maximum, pH 8: about 35% of activity maximum with 5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate, about 65% of activity maximum with 5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein, oxidized acceptor in reverse reaction | Rattus norvegicus | |
FADH2 | flavoprotein, reduced acceptor in forward reaction | Rattus norvegicus | |
NADH | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | 0.051 | (+)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate | - |
Rattus norvegicus |