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Literature summary for 1.5.1.20 extracted from
- Is reduced ferredoxin the physiological electron donor for MetVF-type methylenetetrahydrofolate reductases in acetogenesis? A hypothesis (2022), Int. Microbiol., 25, 7588 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene metVF | Clostridium ljungdahlii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Clostridium ljungdahlii | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | the enzyme dimer has 14.9 mol iron per mol enzyme, it possesses two 4Fe-4S-clusters | Clostridium ljungdahlii |  |
| additional information | the enzyme dimer has 16.2 mol acid-labile sulfur per mol enzyme | Clostridium ljungdahlii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 106000 | - |
analytical gel filtration | Clostridium ljungdahlii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + ferredoxin | Clostridium ljungdahlii | - |
5-methyltetrahydrofolate + reduced ferredoxin | - |
r | |
| 5,10-methylenetetrahydrofolate + ferredoxin | Clostridium ljungdahlii DSM 13528 | - |
5-methyltetrahydrofolate + reduced ferredoxin | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium ljungdahlii | A0A162J7F3 | - |
- |
| Clostridium ljungdahlii DSM 13528 | A0A162J7F3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 57.6fold from cytoplasm by anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration | Clostridium ljungdahlii |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H + H+ | MTHFR is proposed to use the mechanism of flavin-based electron bifurcation | Clostridium ljungdahlii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 156.1 | - |
purified native enzyme, pH 7.0, 37°C | Clostridium ljungdahlii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + ferredoxin | - |
Clostridium ljungdahlii | 5-methyltetrahydrofolate + reduced ferredoxin | - |
r | |
| 5,10-methylenetetrahydrofolate + ferredoxin | - |
Clostridium ljungdahlii DSM 13528 | 5-methyltetrahydrofolate + reduced ferredoxin | - |
r | |
| additional information | reduced methyl viologen is used as electron donor and the MVred:methylene-THF oxidoreductase activity is followed during the purification procedure. Analysis of carbon and electron flow during acetogenesis from H2 + CO2 or CO | Clostridium ljungdahlii | ? | - |
- |
|
| additional information | reduced methyl viologen is used as electron donor and the MVred:methylene-THF oxidoreductase activity is followed during the purification procedure. Analysis of carbon and electron flow during acetogenesis from H2 + CO2 or CO | Clostridium ljungdahlii DSM 13528 | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | (1 * 35000 + 1 * 23000)2, SDS-PAGE | Clostridium ljungdahlii |
| More | the MetVF-type enzyme is a dimer consisting of two of MetVF heterodimers | Clostridium ljungdahlii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methylenetetrahydrofolate reductase | - |
Clostridium ljungdahlii |
| MetVF-type methylenetetrahydrofolate reductase | - |
Clostridium ljungdahlii |
| MTHFR | - |
Clostridium ljungdahlii |
| type II-MTHFR | - |
Clostridium ljungdahlii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Clostridium ljungdahlii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | the activity is relatively stable between 37°C and 50°C but decreases to 56% at 20°C and 23% at 80°C | Clostridium ljungdahlii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Clostridium ljungdahlii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | 2% activity remain at pH 5.0, and 6% activity at pH 9.0 | Clostridium ljungdahlii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Ferredoxin | physiological cofactor | Clostridium ljungdahlii | |
| FMN | the enzyme contains 1.87 mol FMN per mol dimeric heterodimer. MetF binds the flavin | Clostridium ljungdahlii | |
| additional information | NADH and NADPH are not used as electron donor, but reduced ferredoxin is. The enzyme possesses two predicted 4Fe-4S-clusters in MetV | Clostridium ljungdahlii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | depending on the electron donor for methylene-THF reduction, different amounts of ATP can be synthesized | Clostridium ljungdahlii |
| physiological function | possible role of the enzyme in acetogenesis, overview | Clostridium ljungdahlii |
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