BRENDA - Enzyme Database
show all sequences of 1.5.1.21

The putative malate/lactate dehydrogenase from Pseudomonas putida is an NADPH-dependent DELTA1-piperideine-2-carboxylate/DELTA1-pyrroline-2-carboxylate reductase involved in the catabolism of D-lysine and D-proline

Muramatsu, H.; Mihara, H.; Kakutani, R.; Yasuda, M.; Ueda, M.; Kurihara, T.; Esaki, N.; J. Biol. Chem. 280, 5329-5335 (2005)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
DNA sequence determination and analysis, overexpression in Escherichia coli
Pseudomonas putida
Engineering
Protein Variants
Commentary
Organism
additional information
dpk disruption mutant is unable to grow on D-lysine or D-proline as sole carbon source
Pseudomonas putida
Inhibitors
Inhibitors
Commentary
Organism
Structure
NADPH
inhibits the reverse reaction
Pseudomonas putida
pyrroline-2-carboxylate
inhibits the reverse reaction
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Pseudomonas putida
0.034
-
NADP+
pH 10.0, 35°C, with substrate L-proline
Pseudomonas putida
0.034
-
NADPH
pH 8.0, 35°C, with substrate DELTA1-pyrroline-2-carboxylate
Pseudomonas putida
0.14
-
NADP+
pH 10.0, 35°C, with substrate L-pipecolate
Pseudomonas putida
0.14
-
NADPH
pH 8.0, 35°C, with substrate DELTA1-piperideine-2-carboxylate
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Pseudomonas putida
enzyme is involved in the catabolism of D-lysine and D-proline, pathway overview
?
-
-
?
additional information
Pseudomonas putida ATCC 12633
enzyme is involved in the catabolism of D-lysine and D-proline, pathway overview
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas putida
Q5FB93
strain ATCC12633, gene dpkA
-
Pseudomonas putida ATCC 12633
Q5FB93
strain ATCC12633, gene dpkA
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant enzyme from Escherichia coli, to homogeneity
Pseudomonas putida
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
specificity for carbon sources, wild-type shows the highest growth rate on D-proline, the deletion mutant on L-lysine, overview
Pseudomonas putida
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Pseudomonas putida
0.33
-
purified recombinant enzyme, substrates DELTA1-pyrroline-2-carboxylate and NADH
Pseudomonas putida
0.67
-
purified recombinant enzyme, substrates DELTA1-piperideine-2-carboxylate and NADH
Pseudomonas putida
1.4
-
purified recombinant enzyme, substrates L-pipecolate and NADP+
Pseudomonas putida
51
-
purified recombinant enzyme, substrates DELTA1-pyrroline-2-carboxylate and NADPH
Pseudomonas putida
92
-
purified recombinant enzyme, substrates DELTA1-piperideine-2-carboxylate and NADPH
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
DELTA1-piperideine-2-carboxylate + NADPH
NADH is a poor electron donor, lower activity in the reverse reaction direction
656290
Pseudomonas putida
L-pipecolate + NADP+
-
-
-
r
DELTA1-piperideine-2-carboxylate + NADPH
NADH is a poor electron donor, lower activity in the reverse reaction direction
656290
Pseudomonas putida ATCC 12633
L-pipecolate + NADP+
-
-
-
r
DELTA1-pyrroline-2-carboxylate + NADPH
NADH is a poor electron donor, lower activity in the reverse reaction direction
656290
Pseudomonas putida
L-proline + NADP+
-
-
-
r
DELTA1-pyrroline-2-carboxylate + NADPH
NADH is a poor electron donor, lower activity in the reverse reaction direction
656290
Pseudomonas putida ATCC 12633
L-proline + NADP+
-
-
-
r
additional information
enzyme is involved in the catabolism of D-lysine and D-proline, pathway overview
656290
Pseudomonas putida
?
-
-
-
?
additional information
substrate specificity, enzyme shows no malate or lactate dehydrogenase activity
656290
Pseudomonas putida
?
-
-
-
?
additional information
enzyme is involved in the catabolism of D-lysine and D-proline, pathway overview
656290
Pseudomonas putida ATCC 12633
?
-
-
-
?
additional information
substrate specificity, enzyme shows no malate or lactate dehydrogenase activity
656290
Pseudomonas putida ATCC 12633
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
DELTA1-piperideine-2-carboxylate/DELTA1-pyrroline-2-carboxylate reductase
-
Pseudomonas putida
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
assay at
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at, forward reaction
Pseudomonas putida
10
-
assay at, reverse reaction
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
dependent on
Pseudomonas putida
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
substrate and product inhibition kinetics, overview
Pseudomonas putida
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA sequence determination and analysis, overexpression in Escherichia coli
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
dependent on
Pseudomonas putida
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
dpk disruption mutant is unable to grow on D-lysine or D-proline as sole carbon source
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NADPH
inhibits the reverse reaction
Pseudomonas putida
pyrroline-2-carboxylate
inhibits the reverse reaction
Pseudomonas putida
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
substrate and product inhibition kinetics, overview
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Pseudomonas putida
0.034
-
NADP+
pH 10.0, 35°C, with substrate L-proline
Pseudomonas putida
0.034
-
NADPH
pH 8.0, 35°C, with substrate DELTA1-pyrroline-2-carboxylate
Pseudomonas putida
0.14
-
NADP+
pH 10.0, 35°C, with substrate L-pipecolate
Pseudomonas putida
0.14
-
NADPH
pH 8.0, 35°C, with substrate DELTA1-piperideine-2-carboxylate
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Pseudomonas putida
enzyme is involved in the catabolism of D-lysine and D-proline, pathway overview
?
-
-
?
additional information
Pseudomonas putida ATCC 12633
enzyme is involved in the catabolism of D-lysine and D-proline, pathway overview
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme from Escherichia coli, to homogeneity
Pseudomonas putida
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
specificity for carbon sources, wild-type shows the highest growth rate on D-proline, the deletion mutant on L-lysine, overview
Pseudomonas putida
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Pseudomonas putida
0.33
-
purified recombinant enzyme, substrates DELTA1-pyrroline-2-carboxylate and NADH
Pseudomonas putida
0.67
-
purified recombinant enzyme, substrates DELTA1-piperideine-2-carboxylate and NADH
Pseudomonas putida
1.4
-
purified recombinant enzyme, substrates L-pipecolate and NADP+
Pseudomonas putida
51
-
purified recombinant enzyme, substrates DELTA1-pyrroline-2-carboxylate and NADPH
Pseudomonas putida
92
-
purified recombinant enzyme, substrates DELTA1-piperideine-2-carboxylate and NADPH
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
DELTA1-piperideine-2-carboxylate + NADPH
NADH is a poor electron donor, lower activity in the reverse reaction direction
656290
Pseudomonas putida
L-pipecolate + NADP+
-
-
-
r
DELTA1-piperideine-2-carboxylate + NADPH
NADH is a poor electron donor, lower activity in the reverse reaction direction
656290
Pseudomonas putida ATCC 12633
L-pipecolate + NADP+
-
-
-
r
DELTA1-pyrroline-2-carboxylate + NADPH
NADH is a poor electron donor, lower activity in the reverse reaction direction
656290
Pseudomonas putida
L-proline + NADP+
-
-
-
r
DELTA1-pyrroline-2-carboxylate + NADPH
NADH is a poor electron donor, lower activity in the reverse reaction direction
656290
Pseudomonas putida ATCC 12633
L-proline + NADP+
-
-
-
r
additional information
enzyme is involved in the catabolism of D-lysine and D-proline, pathway overview
656290
Pseudomonas putida
?
-
-
-
?
additional information
substrate specificity, enzyme shows no malate or lactate dehydrogenase activity
656290
Pseudomonas putida
?
-
-
-
?
additional information
enzyme is involved in the catabolism of D-lysine and D-proline, pathway overview
656290
Pseudomonas putida ATCC 12633
?
-
-
-
?
additional information
substrate specificity, enzyme shows no malate or lactate dehydrogenase activity
656290
Pseudomonas putida ATCC 12633
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
assay at
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at, forward reaction
Pseudomonas putida
10
-
assay at, reverse reaction
Pseudomonas putida
Other publictions for EC 1.5.1.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743355
Hallen
Reciprocal control of thyroid ...
Canis lupus familiaris, Sus scrofa, Mus musculus, Homo sapiens, Rattus norvegicus, Ovis aries
Neurochem. Res.
42
217-243
2017
-
-
-
-
-
-
6
-
6
-
-
13
-
6
-
-
-
-
-
20
-
-
19
-
18
-
-
-
-
-
-
-
8
-
-
-
-
-
-
8
-
-
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-
6
-
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6
-
-
13
-
-
-
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-
20
-
-
19
-
-
-
-
-
-
-
-
-
-
18
18
-
-
-
742531
Watanabe
Identification and characteri ...
Pseudomonas aeruginosa
FEBS Open Bio
4
240-250
2014
-
-
1
-
-
-
-
6
-
-
1
4
-
1
-
-
-
-
-
1
6
-
12
1
6
1
-
-
6
2
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
6
-
-
1
4
-
-
-
-
-
1
6
-
12
1
1
-
-
6
2
-
-
-
-
2
2
-
6
6
741580
Hallen
Lysine metabolism in mammalia ...
Bos taurus, Mus musculus, Rattus norvegicus, Sus scrofa
Amino Acids
45
1249-1272
2013
-
-
-
-
-
-
-
-
8
-
2
8
-
4
-
-
2
-
-
5
-
-
8
1
12
-
-
-
-
5
-
-
6
-
-
-
-
-
-
6
-
-
-
-
-
-
-
8
-
2
8
-
-
-
2
-
5
-
-
8
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
724927
Umair
Lysine catabolism in Haemonchu ...
Haemonchus contortus, Teladorsagia circumcincta
Exp. Parasitol.
131
101-106
2012
-
-
-
-
-
-
-
2
-
-
-
4
-
6
-
-
-
-
-
4
-
-
4
-
4
-
-
-
-
2
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
2
-
-
-
4
-
-
-
-
-
4
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
672675
Muramatsu
Enzymatic synthesis of L-pipec ...
Pseudomonas putida
Biosci. Biotechnol. Biochem.
70
2296-2298
2006
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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1
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
656290
Muramatsu
The putative malate/lactate de ...
Pseudomonas putida, Pseudomonas putida ATCC 12633
J. Biol. Chem.
280
5329-5335
2005
-
-
1
-
1
-
2
5
-
-
-
2
-
6
-
-
1
-
-
1
6
-
8
-
1
1
-
-
-
2
-
-
1
1
-
-
-
-
1
1
-
1
-
-
2
1
5
-
-
-
2
-
-
-
1
-
1
6
-
8
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
674509
Goto
Crystal structures of DELTA1-p ...
Pseudomonas syringae
J. Biol. Chem.
280
40875-40884
2005
-
-
-
1
-
-
2
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
8
1
1
1
-
1
-
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-
2
-
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-
-
-
2
1
-
-
-
2
-
-
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-
1
-
-
-
-
-
-
-
3
-
8
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
392171
Cao
The OCT plasmid encodes D-lysi ...
Pseudomonas putida
Plasmid
30
83-89
1993
-
-
-
-
-
-
-
-
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-
-
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1
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1
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1
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1
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1
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-
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-
-
-
-
-
392169
Payton
DELTA1-Piperideine-2-carboxyla ...
Pseudomonas putida
J. Bacteriol.
149
864-871
1982
3
-
-
-
-
6
11
4
-
-
1
1
-
3
-
-
1
-
-
-
1
1
5
-
-
1
-
-
-
1
-
-
2
-
-
-
3
-
-
2
-
-
6
-
11
-
4
-
-
1
1
-
-
-
1
-
-
1
1
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
2133
Chang
D-lysine catabolic pathway in ...
Pseudomonas putida
J. Bacteriol.
117
753-764
1974
-
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1
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1
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1
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1
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1
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