BRENDA - Enzyme Database show
show all sequences of 1.5.1.24

The gene CBO0515 from Clostridium botulinum strain Hall A encodes the rare enzyme N5-(carboxyethyl) ornithine synthase, EC 1.5.1.24

Thompson, J.; Hill, K.K.; Smith, T.J.; Pikis, A.; J. Bacteriol. 192, 1151-1155 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Clostridium botulinum
Engineering
Amino acid exchange
Commentary
Organism
D114A
severe reduction (higher than 95%) in enzymatic activity
Clostridium botulinum
E13A
severe reduction (higher than 95%) in enzymatic activity
Clostridium botulinum
H92L
severe reduction (higher than 95%) in enzymatic activity
Clostridium botulinum
K71R
severe reduction (higher than 95%) in enzymatic activity
Clostridium botulinum
R15K
severe reduction (higher than 95%) in enzymatic activity
Clostridium botulinum
W90S
severe reduction (higher than 95%) in enzymatic activity
Clostridium botulinum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
pyruvate
pH 7.5, temperature not specified in the publication, Vmax: 0.9 micromol/min/mg protein
Clostridium botulinum
2.95
-
NADPH
pH 7.5, temperature not specified in the publication
Clostridium botulinum
29.5
-
L-ornithine
pH 7.5, temperature not specified in the publication
Clostridium botulinum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35850
-
calculated from cDNA
Clostridium botulinum
36000
-
SDS-PAGE
Clostridium botulinum
125000
-
gel filtration
Clostridium botulinum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium botulinum
A5HZ59
UniProt
-
Purification (Commentary)
Commentary
Organism
using anion-exchange and phosphocellulose P-11 chromatography
Clostridium botulinum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
6.6
-
pH 7.5, temperature not specified in the publication
Clostridium botulinum
Storage Stability
Storage Stability
Organism
-20C, 20% glycerol, freezing and thawing results in loss of all enzymatic activity
Clostridium botulinum
-20C, 25% (NH4)2SO4, several months, no loss in activity
Clostridium botulinum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-N-acetylornithine + pyruvate + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
D-lysine + pyruvate + NADPH + H+
enzyme also reacts with D-isomer
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
D-ornithine + pyruvate + NADPH + H+
enzyme also reacts with D-isomer
712294
Clostridium botulinum
N5-(L-1-carboxyethyl)-D-ornithine + NADP+ + H2O
-
-
-
?
L-lysine + pyruvate + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
L-ornithine + 2-oxo-butanoic acid + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
L-ornithine + 3-fluoropyruvic acid + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
L-ornithine + 3-mercaptopyruvic acid + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
L-ornithine + glyoxylic acid + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
L-ornithine + methylglyoxal + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
L-ornithine + oxaloacetic acid + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
L-ornithine + pyruvate + NADPH + H+
highest reaction rate
712294
Clostridium botulinum
N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
-
-
?
N2-methylornithine + pyruvate + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
N6-hydroxy-L-lysine + pyruvate + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
O-(2-aminoethyl)-L-serine + pyruvate + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
S-(2-aminoethyl)-L- cysteine + pyruvate + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
in solution, gel filtration
Clostridium botulinum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Clostridium botulinum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Clostridium botulinum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D114A
severe reduction (higher than 95%) in enzymatic activity
Clostridium botulinum
E13A
severe reduction (higher than 95%) in enzymatic activity
Clostridium botulinum
H92L
severe reduction (higher than 95%) in enzymatic activity
Clostridium botulinum
K71R
severe reduction (higher than 95%) in enzymatic activity
Clostridium botulinum
R15K
severe reduction (higher than 95%) in enzymatic activity
Clostridium botulinum
W90S
severe reduction (higher than 95%) in enzymatic activity
Clostridium botulinum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
pyruvate
pH 7.5, temperature not specified in the publication, Vmax: 0.9 micromol/min/mg protein
Clostridium botulinum
2.95
-
NADPH
pH 7.5, temperature not specified in the publication
Clostridium botulinum
29.5
-
L-ornithine
pH 7.5, temperature not specified in the publication
Clostridium botulinum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35850
-
calculated from cDNA
Clostridium botulinum
36000
-
SDS-PAGE
Clostridium botulinum
125000
-
gel filtration
Clostridium botulinum
Purification (Commentary) (protein specific)
Commentary
Organism
using anion-exchange and phosphocellulose P-11 chromatography
Clostridium botulinum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
6.6
-
pH 7.5, temperature not specified in the publication
Clostridium botulinum
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 20% glycerol, freezing and thawing results in loss of all enzymatic activity
Clostridium botulinum
-20C, 25% (NH4)2SO4, several months, no loss in activity
Clostridium botulinum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-N-acetylornithine + pyruvate + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
D-lysine + pyruvate + NADPH + H+
enzyme also reacts with D-isomer
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
D-ornithine + pyruvate + NADPH + H+
enzyme also reacts with D-isomer
712294
Clostridium botulinum
N5-(L-1-carboxyethyl)-D-ornithine + NADP+ + H2O
-
-
-
?
L-lysine + pyruvate + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
L-ornithine + 2-oxo-butanoic acid + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
L-ornithine + 3-fluoropyruvic acid + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
L-ornithine + 3-mercaptopyruvic acid + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
L-ornithine + glyoxylic acid + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
L-ornithine + methylglyoxal + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
L-ornithine + oxaloacetic acid + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
L-ornithine + pyruvate + NADPH + H+
highest reaction rate
712294
Clostridium botulinum
N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
-
-
?
N2-methylornithine + pyruvate + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
N6-hydroxy-L-lysine + pyruvate + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
O-(2-aminoethyl)-L-serine + pyruvate + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
S-(2-aminoethyl)-L- cysteine + pyruvate + NADPH + H+
weak reaction rate
712294
Clostridium botulinum
? + NADP+ + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
in solution, gel filtration
Clostridium botulinum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Clostridium botulinum
Other publictions for EC 1.5.1.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712294
Thompson
The gene CBO0515 from Clostrid ...
Clostridium botulinum
J. Bacteriol.
192
1151-1155
2010
-
-
1
-
6
-
-
3
-
-
3
-
-
2
-
-
1
-
-
-
1
2
15
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
3
-
-
3
-
-
-
-
1
-
-
1
2
15
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
392177
Ruvinov
Tetrameric N5-(L-1-carboxyethy ...
Lactococcus lactis
Arch. Biochem. Biophys.
371
115-123
1999
-
1
-
-
-
1
-
-
-
-
13
-
-
1
-
-
1
-
-
-
-
-
2
1
-
-
1
-
-
-
-
1
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
13
-
-
-
-
1
-
-
-
-
2
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
392178
Sackett
N5-(L-1-carboxyethyl)-L-ornith ...
Lactococcus lactis
Protein Sci.
8
2121-2129
1999
-
1
1
1
-
-
-
-
-
-
4
-
-
3
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392176
Donkersloot
Cloning, expression, sequence ...
Lactococcus lactis, Lactococcus lactis K1-23
J. Biol. Chem.
270
12226-12234
1995
-
2
1
-
-
-
-
-
-
-
1
-
-
6
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392175
Miller
Enzymatic synthesis and charac ...
Lactococcus lactis
Amino Acids
6
189-198
1994
-
1
1
-
-
-
2
1
1
-
1
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
2
-
1
1
-
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392174
Thompson
Transposon-encoded sucrose met ...
Lactococcus lactis, Lactococcus lactis K1-23
J. Biol. Chem.
266
14573-14579
1991
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392173
Donkersloot
Simultaneous loss of N5-(carbo ...
Lactococcus lactis
J. Bacteriol.
172
4122-4126
1990
-
-
1
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392172
Thompson
N5-(L-1-carboxyethyl)-L-ornith ...
Lactococcus lactis
J. Biol. Chem.
264
9592-9601
1989
-
-
-
-
-
1
1
4
-
-
5
-
-
1
-
-
1
-
-
-
1
1
3
2
-
1
2
-
1
1
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
4
-
-
5
-
-
-
-
1
-
-
1
1
3
2
-
1
2
-
1
1
-
-
-
-
-
-
-
-