BRENDA - Enzyme Database
show all sequences of 1.5.1.26

Occurrence of beta-alanine-specific opine dehydrogenase in the muscle of the limpet Cellana grata Gould (Archaeogastropoda)

Kan-no, N.; Sato, M.; Yokoyama, T.; Nagahisa, E.; Comp. Biochem. Physiol. B 123B, 125-136 (1999)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.032
-
NADH
pH 7.1, 30C
Cellana grata
0.078
-
NAD+
pH 9.0, 30C
Cellana grata
0.19
-
pyruvate
pH 7.1, 30C
Cellana grata
14.3
-
beta-Alanine
pH 7.1, 30C
Cellana grata
35.2
-
beta-alanopine
pH 9.0, 30C
Cellana grata
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39700
-
gel filtration
Cellana grata
40800
-
x * 40800, SDS-PAGE
Cellana grata
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
beta-alanine + pyruvate + NADH
Cellana grata
specific for beta-alanine
beta-alanopine + NAD+ + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Cellana grata
-
Gold, isozyme beta-AlDH-2
-
Purification (Commentary)
Purification (Commentary)
Organism
native enzyme by ammonium sulfate fractionation and several chromatographic steps, 705.6fold
Cellana grata
Source Tissue
Source Tissue
Commentary
Organism
Textmining
muscle
-
Cellana grata
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Cellana grata
13
-
purified enzyme, reverse reaction
Cellana grata
190.5
-
purified enzyme, forward reaction
Cellana grata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
beta-alanine + pyruvate + NADH
specific for beta-alanine
655255
Cellana grata
beta-alanopine + NAD+ + H2O
-
-
-
?
additional information
tauropine, alanopine, strombine, and octopine are poor substrates
655255
Cellana grata
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
x * 40800, SDS-PAGE
Cellana grata
Synonyms
Synonyms
Commentary
Organism
beta-AlDH-2
-
Cellana grata
More
the enzyme belongs to the opine dehydrogenase family of enzymes
Cellana grata
OpDH
-
Cellana grata
opine dehydrogenase
-
Cellana grata
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Cellana grata
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.1
-
opine biosynthetic reaction direction
Cellana grata
9
-
catabolic reaction direction
Cellana grata
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
10
half-maximal forward reaction at pH 5.8, half-maximal reverse reaction at pH 8.5
Cellana grata
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Cellana grata
NADH
-
Cellana grata
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Cellana grata
NADH
-
Cellana grata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.032
-
NADH
pH 7.1, 30C
Cellana grata
0.078
-
NAD+
pH 9.0, 30C
Cellana grata
0.19
-
pyruvate
pH 7.1, 30C
Cellana grata
14.3
-
beta-Alanine
pH 7.1, 30C
Cellana grata
35.2
-
beta-alanopine
pH 9.0, 30C
Cellana grata
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39700
-
gel filtration
Cellana grata
40800
-
x * 40800, SDS-PAGE
Cellana grata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
beta-alanine + pyruvate + NADH
Cellana grata
specific for beta-alanine
beta-alanopine + NAD+ + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme by ammonium sulfate fractionation and several chromatographic steps, 705.6fold
Cellana grata
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
muscle
-
Cellana grata
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Cellana grata
13
-
purified enzyme, reverse reaction
Cellana grata
190.5
-
purified enzyme, forward reaction
Cellana grata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
beta-alanine + pyruvate + NADH
specific for beta-alanine
655255
Cellana grata
beta-alanopine + NAD+ + H2O
-
-
-
?
additional information
tauropine, alanopine, strombine, and octopine are poor substrates
655255
Cellana grata
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
x * 40800, SDS-PAGE
Cellana grata
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Cellana grata
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.1
-
opine biosynthetic reaction direction
Cellana grata
9
-
catabolic reaction direction
Cellana grata
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
10
half-maximal forward reaction at pH 5.8, half-maximal reverse reaction at pH 8.5
Cellana grata
Other publictions for EC 1.5.1.26
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725659
Lee
-
The enzyme activities of opine ...
Meretrix lusoria
J. Mar. Sci. Technol.
19
361-367
2011
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1
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1
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1
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4
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1
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1
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1
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1
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4
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1
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-
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1
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1
-
-
655255
Kan-no
-
Occurrence of beta-alanine-spe ...
Cellana grata
Comp. Biochem. Physiol. B
123B
125-136
1999
-
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-
-
-
-
-
5
-
-
2
1
-
1
-
-
1
-
-
1
3
-
2
1
4
1
-
-
-
2
1
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2
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2
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5
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2
1
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1
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1
3
-
2
1
1
-
-
-
2
1
-
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-
-
-
13291
Hammen
-
Opine oxidoreductases in marin ...
Glycera sp., no activity in Cerebratulus sp., no activity in Chaetopterus variopedatus, no activity in Clymenella torquata, no activity in Hydroides sp., no activity in Lepidonotus sp., no activity in Lineus sp., no activity in Nereis sp., no activity in Priapulus sp., no activity in Urechis sp., Phascolopsis sp., Phascolosoma sp.
Comp. Biochem. Physiol. B
106
989-992
1993
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12
-
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-
-
-
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3
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3
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13292
Sato
-
Distribution of opine dehydrog ...
Anthopleura japonica, Anthopleura nigrescens, Buccinum isaotakii, Cellana grata, Azumapecten farreri nipponensis, Chlorostoma lischkei, Crassostrea gigas, Fusitriton oregonensis, Haliotis discus hannai, Hemigrapsus sanguineus, Littorina brevicula, Meretrix lusoria, Mytilus edulis, Neptunea arthritica, no activity in Aplysia kurodai, no activity in Aplysia juliana, no activity in Asterias amurensis, no activity in Asterina pectinifera, no activity in Aurelia aurita, no activity in Balanus cariosus, no activity in Halichondria japonica, no activity in Halocynthia roretzi, no activity in Hexagrammos otakii, no activity in Liolophura japonica, no activity in Loligo bleekeri, no activity in Octopus membranaceus, no activity in Octopus vulgaris, no activity in Oncorhynchus keta, no activity in Pagurus samuelis, no activity in Pugettia quadridens, no activity in Stichopus japonicus, no activity in Strongylocentrotus nudus, no activity in Todarodes pacificus, Mizuhopecten yessoensis, Perinereis nuntia, Capitulum mitella, Pseudopotamilla occelata, Reishia clavigera, Ruditapes philippinarum, Anadara broughtonii, Solaster paxillatus, Scelidotoma gigas, Pseudocardium sachalinense
Comp. Biochem. Physiol. B
106
955-960
1993
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43
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29
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24
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29
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24
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13300
Hammen
-
Opine oxidoreductases in brach ...
Amphitrite sp., Lunarca ovalis, Crassostrea virginica, Glottidia pyramidata, no activity in Crepidula fornicata, no activity in Dentalium pilsbryi, no activity in Haliotis rufescens, no activity in Littorina littorea, no activity in Lyonsia hyalina, no activity in Membranipora tenuis, no activity in Mopalia muscosa, no activity in Mya arenaria, no activity in Nucella lapillus, no activity in Nucula proxima, no activity in Solemya velum, no activity in Spisula solidissima, no activity in Tegula funebralis, no activity in Terebratalia transversa, Testudinalia testudinalis, Themiste sp., Urosalpinx cinerea
Biochem. Syst. Ecol.
19
263-269
1991
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21
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7
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7
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644725
Sato
-
Isolation of a new opine, beta ...
Anadara broughtonii
Comp. Biochem. Physiol. B
88B
803-806
1987
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1
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5
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1
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5
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1
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