Literature summary for 1.5.1.3 extracted from

Stone, S.R.; Morrison, J.F.
Dihydrofolate reductase from Escherichia coli: the kinetic mechanism with NADPH and reduced acetylpyridine adenine dinucleotide phosphate as substrates (1988), Biochemistry, 27, 5493-5499.

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Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+	kinetic mechanism	Escherichia coli	a
5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+	rapid random equilibrium mechanism with NADPH and reduced acetylpyridine adenine nucleotide as cofactor	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7,8-dihydrofolate + acetylpyridine adenine nucleotide, reduced	-	Escherichia coli	5,6,7,8-tetrahydrofolate + acetylpyridine adenine nucleotide, oxidized	-	?
additional information	3-acetylpyridine adenine dinucleotide + dihydrofolate: rapid equilibrium random mechanism	Escherichia coli	?	-	?
additional information	NADPH + dihydrofolate: steady-state random mechanism	Escherichia coli	?	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2	-	7,8-dihydrofolate	with acetylpyridine adenine nucleotide	Escherichia coli
2	-	acetylpyridine adenine nucleotide	with 7,8-dihydrofolate	Escherichia coli
11	-	NADPH	with 7,8-dihydrofolate	Escherichia coli
18	-	7,8-dihydrofolate	with NADPH	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
3-acetylpyridine adenine nucleotide	-	Escherichia coli
NADPH	-	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	Ki of substrates dihydrofolate, NADPH and acetylpyridine adenine nucleotide	Escherichia coli

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Release 2023.1 (January 2023)