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Literature summary for 1.5.1.3 extracted from
- The occurence and properties of pteridine reductase: dihydrofolate reductase in Crithidia fasciculata (1983), Agric. Biol. Chem., 47, 251-258.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chaotrophes | e.g. guanidine-HCl; formamide; urea | Crithidia fasciculata | |
| folate | - |
Crithidia fasciculata |  |
| L-erythro-biopterin | - |
Crithidia fasciculata | |
| methotrexate | - |
Crithidia fasciculata | |
| N-ethylmaleimide | - |
Crithidia fasciculata | |
| NADP+ | - |
Crithidia fasciculata | |
| p-chloromercuribenzoate | - |
Crithidia fasciculata | |
| pyrimethamine | - |
Crithidia fasciculata | |
| trimethoprim | - |
Crithidia fasciculata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0009 | - |
dihydropteroate | - |
Crithidia fasciculata | |
| 0.0021 | - |
NADPH | with dihydropteroate | Crithidia fasciculata | |
| 0.0034 | - |
6-Hydroxymethylpterin | - |
Crithidia fasciculata | |
| 0.0035 | - |
L-threo-neopterin | - |
Crithidia fasciculata | |
| 0.0048 | - |
7,8-dihydrofolate | - |
Crithidia fasciculata | |
| 0.0059 | - |
NADPH | with 7,8-dihydrofolate or 6-hydroxymetylpterin | Crithidia fasciculata | |
| 0.011 | - |
NADPH | with L-threo-neopterin | Crithidia fasciculata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
gel filtration | Crithidia fasciculata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crithidia fasciculata | - |
bifunctional enzyme: pteridine reductase-dihydrofolate reductase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| bifunctional enzyme: pteridine reductase-dihydrofolate reductase | Crithidia fasciculata |
Storage Stability
| Storage Stability | Organism |
|---|---|
| ammonium sulfate, 55% saturation, 0°C, for at least 1 year | Crithidia fasciculata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-hydroxymethylpterin + NADPH | - |
Crithidia fasciculata | 6-hydroxymethyl-7,8-dihydropterin + NADP+ | - |
? | |
| 6-methylpterin + NADPH | - |
Crithidia fasciculata | 6-methyl-7,8-dihydropterin + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | - |
Crithidia fasciculata | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| D-erythro-7,8-neopterin + NADPH | low activity | Crithidia fasciculata | ? | - |
? | |
| D-threo-neopterin + NADPH | low activity | Crithidia fasciculata | ? | - |
? | |
| dihydropteroate + NADPH | - |
Crithidia fasciculata | ? | - |
? | |
| folate + NADPH | no activity | Crithidia fasciculata | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| L-erythro-neopterin + NADPH | - |
Crithidia fasciculata | ? | - |
? | |
| L-threo-7,8-dihydroneopterin + NADPH | low activity | Crithidia fasciculata | ? | - |
? | |
| L-threo-neopterin + NADPH | - |
Crithidia fasciculata | ? | - |
? | |
| additional information | no activity with folate, sepiapterin, isoxanthopterin, leucopterin | Crithidia fasciculata | ? | - |
? | |
| pterin + NADPH | low activity | Crithidia fasciculata | 7,8-dihydropterin + NADP+ | - |
? | |
| xanthopterin + NADPH | low activity | Crithidia fasciculata | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Crithidia fasciculata |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | not | Crithidia fasciculata | |
| NADPH | - |
Crithidia fasciculata | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00034 | - |
L-erythro-biopterin | with 7,8-dihydrofolate | Crithidia fasciculata | |
| 0.00072 | - |
pyrimethamine | with 7,8-dihydrofolate | Crithidia fasciculata | |
| 0.0011 | - |
methotrexate | with 7,8-dihydrofolate | Crithidia fasciculata | |
| 0.0064 | - |
trimethoprim | with 7,8-dihydrofolate | Crithidia fasciculata | |
| 0.021 | - |
NADP+ | with 7,8-dihydrofolate | Crithidia fasciculata | |
| 0.036 | - |
folate | with 7,8-dihydrofolate | Crithidia fasciculata | |
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