General Stability | Organism |
---|---|
2-mercaptoethanol, 4 mM, stabilizes purified preparation, inclusion in purification steps causes loss of activity | Crithidia fasciculata |
glycerol, 30%, stabilization of purified preparation | Crithidia fasciculata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aminopterin | - |
Crithidia fasciculata | |
chaotrophes | e.g. guanidine-HCl; formamide; urea | Crithidia fasciculata | |
K+ | - |
Crithidia fasciculata | |
methotrexate | - |
Crithidia fasciculata | |
N-ethylmaleimide | - |
Crithidia fasciculata | |
p-chloromercuribenzoate | - |
Crithidia fasciculata | |
pyrimethamine | - |
Crithidia fasciculata | |
trimethoprim | - |
Crithidia fasciculata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
7,8-dihydrofolate | - |
Crithidia fasciculata | |
0.0027 | - |
NADPH | - |
Crithidia fasciculata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
2 * 58000, isoform IIa, SDS-PAGE | Crithidia fasciculata |
110000 | - |
isoform IIa, gel filtration | Crithidia fasciculata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crithidia fasciculata | - |
three different forms I, IIa and IIb | - |
Purification (Comment) | Organism |
---|---|
isoform IIa | Crithidia fasciculata |
Renatured (Comment) | Organism |
---|---|
denaturation with 4 M urea, no renaturation by removing urea by dialysis | Crithidia fasciculata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Crithidia fasciculata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | reverse reaction at only one tenth of forward reaction | Crithidia fasciculata | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 58000, isoform IIa, SDS-PAGE | Crithidia fasciculata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Crithidia fasciculata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | not | Crithidia fasciculata | |
NADPH | - |
Crithidia fasciculata |