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Literature summary for 1.5.1.3 extracted from
- Dihydrofolate reductase from bakers yeast. Purification and properties (1972), Hoppe-Seyler's Z. Physiol. Chem., 353, 773-781.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| folate | - |
Saccharomyces cerevisiae |  |
| iodoacetamide | - |
Saccharomyces cerevisiae | |
| methotrexate | - |
Saccharomyces cerevisiae | |
| N10-formylaminopterin | - |
Saccharomyces cerevisiae | |
| NADP+ | - |
Saccharomyces cerevisiae | |
| p-chloromercuribenzoate | - |
Saccharomyces cerevisiae | |
| trimethoprim | - |
Saccharomyces cerevisiae | |
| Urea | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Saccharomyces cerevisiae | |
| 0.013 | - |
7,8-dihydrofolate | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.75 | - |
purified enzyme | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | equilibrium constant: 320000000000 | Saccharomyces cerevisiae | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 39000, SDS-PAGE | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11.5 | - |
7,8-dihydrofolate | - |
Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | specific | Saccharomyces cerevisiae | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0016 | - |
N10-formyldihydrofolate | - |
Saccharomyces cerevisiae | |
| 0.0027 | - |
trimethoprim | with N10-formyldihydrofolate | Saccharomyces cerevisiae | |
| 0.025 | - |
folate | with 7,8-dihydrofolate | Saccharomyces cerevisiae | |
| 0.3 | - |
NADP+ | with NADPH | Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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