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Literature summary for 1.5.1.3 extracted from

  • Baccanari, D.P.; Tansik, R.L.; Joyner, S.S.; Fling, M.E.; Smith, P.L.
    Characterization of Candida albicans dihydrofolate reductase (1989), J. Biol. Chem., 264, 1100-1107.
    View publication on PubMed

Application

Application Comment Organism
medicine enzyme is a target of several anti-folate inhibitory drugs to combat bacteria, protozoa and cancer Candida albicans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Candida albicans

Inhibitors

Inhibitors Comment Organism Structure
K+
-
Candida albicans
methotrexate
-
Candida albicans
additional information overview Candida albicans
trimethoprim
-
Candida albicans
Urea
-
Candida albicans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Candida albicans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
recombinant from E. coli, gel filtration Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7,8-dihydrofolate + NADPH Candida albicans key enzyme in biosynthesis of purines, pyrimidines and several amino acids 5,6,7,8-tetrahydrofolate + NADP+
-
?
additional information Candida albicans key enzyme in the biosynthesis of purines, pyrimidines and several amino acids ?
-
?

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli Candida albicans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
250
-
purified enzyme Candida albicans

Storage Stability

Storage Stability Organism
4°C, above 1 mg/ml protein concentration, 1 month Candida albicans
4°C, pure, 20% glycerol, several weeks Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrofolate + NADPH key enzyme in biosynthesis of purines, pyrimidines and several amino acids Candida albicans 5,6,7,8-tetrahydrofolate + NADP+
-
?
additional information
-
Candida albicans ?
-
?
additional information key enzyme in the biosynthesis of purines, pyrimidines and several amino acids Candida albicans ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 25000, recombinant from Escherichia coli, SDS-PAGE Candida albicans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
-
Candida albicans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000015
-
methotrexate recombinant from E. coli Candida albicans
0.0043
-
trimethoprim recombinant from E. coli Candida albicans