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Literature summary for 1.5.1.3 extracted from

  • Ma, L.; Kovacs, J.A.
    Expression and characterization of recombinant human-derived Pneumocystis carinii dihydrofolate reductase (2000), Antimicrob. Agents Chemother., 44, 3092-3096.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.3

Cloned(Commentary)

Cloned (Comment) Organism
human-derived gene is expressed in Escherichia coli Pneumocystis carinii

Inhibitors

Inhibitors Comment Organism Structure
methotrexate
-
 Pneumocystis carinii
pyrimethamine poor inhibitor Pneumocystis carinii
trimethoprim poor inhibitor Pneumocystis carinii
trimetrexate
-
 Pneumocystis carinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
 7,8-dihydrofolate recombinant from E. coli Pneumocystis carinii
0.0043
-
 NADPH recombinant from E. coli Pneumocystis carinii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 SDS-PAGE and DNA-sequence determination Pneumocystis carinii

Organism

Organism UniProt Comment Textmining
Pneumocystis carinii
-
 human-derived
-

Purification (Commentary)

Purification (Comment) Organism
recombinant purified from Escherichia coli Pneumocystis carinii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000000016
-
 methotrexate recombinant enzyme derived from E. coli Pneumocystis carinii
0.00000023
-
 trimetrexate recombinant enzyme derived from E. coli Pneumocystis carinii
0.000065
-
 pyrimethamine recombinant enzyme derived from E. coli Pneumocystis carinii
0.00028
-
 trimethoprim recombinant enzyme derived from E. coli Pneumocystis carinii